摘要
【目的】分析心脏脂肪酸结合蛋白(H-FABP)和脂肪细胞型脂肪酸结合蛋白(A-FABP)基因mRNA在不同日龄清远麻鸡不同组织中的差异表达量,为进一步阐明H-FABP、A-FABP在鸡IMF含量和腹脂沉积中的作用机制奠定基础。【方法】依据鸡H-FABP和A-FABP基因及参照基因GAPDH序列设计引物,以鸡心肌、胸肌、腿肌、肝脏和腹脂mRNA逆转录合成的cDNA为模版,应用SYBR GreenⅠ实时荧光定量PCR,检测56和120日龄清远麻鸡A-FABP、H-FABP基因mRNA的差异表达量。【结果】H-FABP、A-FABP和GAPDH的基因融解曲线均为单峰,无杂峰及二聚体,定量准确。120 d时清远麻鸡的胸肌和腿肌的肌内脂肪(IMF)含量显著高于56 d;H-FABP基因mRNA 120 d时在心肌、胸肌和腿肌的差异表达量显著高于56 d,且同一日龄时在心肌的差异表达量显著高于胸肌和腿肌(P<0.05)。120 d时清远麻鸡A-FABP基因mRNA在腹脂和肝脏的差异表达量显著高于56 d;同一日龄时,A-FABP基因mRNA在腹脂的差异表达量显著高于肝脏,A-FABP基因在心肌、胸肌和腿肌中不表达。相关分析表明,H-FABP基因mRNA差异表达量与胸肌、腿肌IMF含量分别呈显著和极显著正相关,A-FABP基因mRNA差异表达量与腹脂率呈极显著负相关。【结论】H-FABP基因主要在鸡肌肉组织的肌内脂肪中表达,而且在心肌中高度表达,与IMF含量呈显著或极显著的正相关,其是IMF含量的下调基因;A-FABP基因主要在腹脂与肝脏中表达,且在腹脂中高度表达,与腹脂率呈极显著负相关,是脂肪细胞的上调基因,且为正调控。
【Objective】 Heart fatty acidbinding protein（H-FABP） and adipocyte fatty acidbinding protein and （A-FABP）gene expression of different tissues were analysed in Qingyuan patridge chicken at different ages.The study will establish a basis for clarifying H-FABP and A-FABP gene expression mechanism in IMF and abdomen fat deposition.【Method】 The primers were designed according to the sequences of H-FABP,A-FABP and GAPDH gene.The c-DNA fragment amplified from leg muscle,breast muscle,cardiac muscle,liver and abdominal fat was used as a standard template;H-FABP and A-FABP gene expression was detected using SYBR Green I realtime fluorescent quantification PCR.The relative expression in H-FABP,A-FABP gene was detected using real time quantitative RT-PCR in Qingyuan patridge chicken.【Result】 Melting curve analysis showed a single peak of the genes.IMF content in breast muscle and leg muscle of Qingyuan partridge on 120 d was higher than that of 56 d.However,the relative H-FABP mRNA level in these tissues was significantly lower than that of Qingyuan partridge on 56 d.The relative H-FABP mRNA level in Cardiac muscle was significantly higher than that of breast muscle and leg muscle.The relative AFABP mRNA levels of abdomen fat and liver were significantly higher than that of the other tissues（P〈0.05）.The relative A-FABP mRNA level in abdomen fat was higher than that in liver.H-FABP mRNA expression level was significantly postive to IMF contents.A-FABP mRNA expression level was significantly negative to abdomen fat and liver.【Conclusion】 H-FABP gene mRNA was expressed in IMF of different muscle tissues.Moreover,the relative H-FABP mRNA level was much higher in Cardiac muscle.H-FABP mRNA expression level was significantly positive to IMF.Furthermore,it had negative effect on IMF content.A-FABP gene mRNA was expressed in abdomen fat and liver.Moreover,the relative A-FABP mRNA levels in abdominal fat was higher.A-FABP mRNA expression level was significantly negative to abdominal fat.A-FABP mRNA expression level had a significantly positive effect on abdominal fat rate.
出处
《西北农林科技大学学报（自然科学版）》
CSCD
北大核心
2010年第4期53-58,共6页
Journal of Northwest A&F University(Natural Science Edition)
基金
国家科技支撑计划项目(2006BDA01A09
2008BADC1B01
2008BADC1B03)
国家"863"计划项目(2008AA101009-7)
作者简介
[作者简介]屠云沽（1974-），女，江苏睢宁人，在读博士，主要从事动物遗传资源评价保护与利用研究。E-mail：tyj3030@126．com.
