摘要
【目的】研究新疆3个地方山羊品种KIF-Ⅰ基因外显子6的多态性及其与产绒性状的相关性,为新疆山羊品种的选育提供依据。【方法】利用PCR-SSCP和DNA序列分析法,对213只新疆山羊、294只南疆绒山羊、253只博格达绒山羊的KIF-Ⅰ基因外显子6的多态性进行检测,并分析其与绒细度、绒厚度、产绒量和产绒后体质量的相关性。【结果】①KIF-Ⅰ基因外显子6区域存在1个突变点,即c.5106位发生了碱基T转变为C(T>C)的突变(M23912:c.5106 T>C)。在3个山羊品种中,检测到了TT、CC、TC 3种基因型,T和C 2个等位基因,其中T为优势等位基因,其在新疆山羊、南疆绒山羊、博格达绒山羊中的频率分别为0.502,0.509和0.583,且3个山羊品种均处于Hardy-Weinberg平衡状态(P>0.05)。②方差分析表明,该多态位点仅与绒细度存在着显著相关性(P<0.05),且CC基因型个体绒细度显著小于TC和TT基因型个体;绒厚度、产绒量和产绒后体质量在3种不同基因型间差异均不显著(P>0.05)。【结论】CC基因型可作为山羊绒细度性状标记辅助选择的有效DNA标记。
【Objective】 This paper was to study the association of polymorphism of cashmere goat KIF-Ⅰgene exon 6 with cashmere production traits so that it could provide a scientific basis for selective breeding.【Method】 PCRSSCP and DNA sequence method were used to detect polymorphism of KIF-Ⅰ gene exon 6 in 213 Xinjiang goats,294 Nanjiang cashmere goats and 253 Bogeda cashmere goats.Correlations between different genotypes and cashmere fineness,down cashmere thickness,cashmere yield,body weight after combing were analyzed.【Result】 ①The result showed the mutation M23912：c.5106 T〉C in exon 6 of KIF-Ⅰ gene.Moreover,we detected three genotypes（TT,CC and TC）,two alleles（T and C）,the allele T was predominant in three populations.The frequencies of allele T in Xinjiang goat,Nanjiang cashmere goat and Bogeda cashmere goat were 0.502,0.509 and 0.583,respectively,which was at HardyWeinberg equilibrium （P〉0.05）.②Moreover,there was a significant correlation between polymorphism and cashmere fineness at this locus.The individual mean fiber diameter for genotype CC was less than that of genotype TT and TC （P〈0.05）.However,in down cashmere thickness,cashmere yield and body weight after combing traits of CC,TC and TT genotypes of the correlation analysis,the results showed no significant difference （P〉0.05）.【Conclusion】 Hence,genotype CC of KIF-Ⅰ gene can be used as a molecular marker for breeding cashmere fineness in goat markerassisted selection.
出处
《西北农林科技大学学报（自然科学版）》
CSCD
北大核心
2010年第4期47-52,共6页
Journal of Northwest A&F University(Natural Science Edition)
基金
新疆维吾尔自治区高校科研计划-科学研究重点项目(20090608-115118343)
作者简介
[作者简介]方光新（1963-），男，内蒙呼和浩特人，推广研究员，主要从事畜牧技术与推广研究。E-mail：FGX848@sohu．com.
[通信作者]刘武军（1966-），女，新疆乌鲁木齐人，副教授，硕士生导师，主要从事反刍动物生产及动物遗传育种研究。E-mail：1wj-WS@163．com.
