摘要
目的探讨STAT3反义寡核苷酸的抗肿瘤机制,为乳腺癌的基因治疗提供新的实验依据。方法应用阳离子脂质体介导STAT3反义寡核苷酸转染乳腺癌MCF-7细胞,在倒置显微镜下观察细胞生长情况及形态变化;采用MTT比色法检测细胞增殖状态;流式细胞仪检测细胞凋亡率及细胞周期;Westernblot检测STAT3总蛋白和p-STAT3蛋白的表达。结果STAT3反义核苷酸转染乳腺癌细胞后,能抑制MCF-7细胞的增殖,促进MCF-7细胞的凋亡,STAT3蛋白的表达和磷酸化水平下降,出现G1期阻滞。结论STAT3反义核苷酸能抑制MCF-7细胞的增殖,促进MCF-7细胞的凋亡,这将为乳腺癌的治疗提供新的方向。
Objective To observe the influences of STAT3 antisense oligonucleotide on proliferation and apoptosis of MCF-7 breast cancer cells. To study the effects of STAT3 antisense oligonucleotides on MCF7 breast cancer cells in ovder to provide a new experiment evidence for breast cancer biological ther apy. Methods The MCF-7 breast canner cell was transfected with STAT3 antisense oligonucleotide mediated by catholyte liposome. The cells growth and morphology were observed with a convert microscope. The proliferation of the cells was evaluated by MTT assay and the cell cycle was determined by flow cytometry. The expressions of total STAT3 protein and p-STAT3 protein were measured by western blot. Results The MCF-7 breast canner cell was transfected with STAT3 antisense oligonucleotide, which led to a sevies results including the inhibition of MCF-7 cell proliferation, the apoptosis of MCF7 cell, the downregulation of STAT3 protein expression and phosphorylation,and the arrest at G1 phase. Conclusion STAT3 antisense oligonucleotides could inhibit MCF-7 cell proliferation, promote MCF-7 cell apoptosis,which may provide a new direction for the treatment of breast cancer.
出处
《肿瘤防治研究》
CAS
CSCD
北大核心
2010年第3期263-265,共3页
Cancer Research on Prevention and Treatment
作者简介
陈佳（1982-），女，硕士在读，主要从事肿瘤的基因治疗的研究
通讯作者： 张蕴莉 E-mail：zyl8546@126.com
