摘要
以血吸虫虫卵浸出液为抗原，金标记兔抗人ＩｇＧ为显示剂，建立了检测抗血吸虫抗体的金标免疫渗滤法（ＤＩＧＦＡ）。以该法检测血吸虫病患者血清８７份，阳性检出率为９８．９％；非流行区血清１００份，阴性符合率为１００％；１５例肺吸虫病患者血清，１例出现阳性反应，交叉反应率为６．７％；１０例华支睾吸虫病患者和２５例乙型肝炎患者，均无交叉反应。本法与ＥＬＩＳＡ比较，符合率达９６．７％。揭示ＤＩＧＦＡ的敏感性和特异性与ＥＬＩＳＡ相近，但方法简便、快速、试剂稳定，且无需特殊设备。
Dot immunogold filtration assay (DIGFA) was established to detect anti schistosome antibodies, using schistosome egg extract as antigen and rabbit anti human IgG labelled with colloidal gold as probe. Eighty six of eighty seven serun samples from patients infected with schistosome were positive. The positive rate was 98.9%. All of 100 sera of control from non endemic areas were negative. Only one out of 15 sera from patients with paragonimiasis showed positive. None of 10 sera from patients with clonorchiasis and 25 sera from patients with HBV were positive. DIGFA and ELISA had a similar sensitivity and specificity. The coincidence rate was 96.7%. However, DIGFA was simpler and easier to conduct and required no instrument. Therefore, this method is applicable for field investigation.
出处
《中国寄生虫病防治杂志》
CSCD
1998年第4期308-310,共3页
Chinese Journal of Parasitic Disease Control
