摘要
目的探讨生长激素（ＧＨ）在免疫调节中的作用。方法以ＮＫ细胞、白细胞介素（ＩＬ）１α和ＩＬ２活性、肿瘤坏死因子（ＴＮＦα）含量及ＴＮＦαｍＲＮＡ表达等为指标，对去垂体大鼠及生长激素缺乏症（ＧＨＤ）患儿在ＧＨ治疗前后的免疫指标进行了观察。结果⑴去垂体鼠淋巴细胞数量、ＮＫ细胞活性、ＩＬ１α和ＩＬ２活性均明显下降，ＧＨ可使上述指标得到不同程度的恢复；⑵去垂体鼠大肠杆菌内毒素诱导的ＴＮＦα合成明显降低，巨噬细胞ＴＮＦαｍＲＮＡ表达亦大幅度下降，ＧＨ可使大肠杆菌内毒素诱导的ＴＮＦα合成增高２．５倍以上，ＴＮＦαｍＲＮＡ表达亦明显增高；⑶ＧＨＤ患儿ＮＫ细胞活性明显降低，经ＧＨ治疗３个月后恢复到正常水平；⑷ＧＨＤ患儿ＩＬ１α和ＩＬ２活性偏低，治疗后两者有逐渐增高的趋势。结论ＧＨ有一定的免疫调节作用。
Objective To study the effect of growth hormone (GH) on immune system. Methods NK cell activity, interleukin 1α (IL 1α) and IL 2 activities, and tumor necrosis factor α (TNFα) and TNFα mRNA levels were measured to evaluate the changes in immune functions in hypophysectomized rats and in growth hormone deficient (CHD) children after GH treatment. Results (1) Hypophysectomized rats exhibited a cellular depletion in spleen, and a reduction in spleen NK cell activity and IL 1α and IL 2 activities. Thyroxine and hydrocortisone did not alter the response of these rats, while a recovery of all the parameters was found with GH in combination. (2) A reduction in macrophage production of TNFα stimulated with lipopolysaccharide (LPS) in vitro and extremly decreased lung macrophage TNFα mRNA levels were observed, which could be markedly increased with GH, but not thyroxine and hydrocortisone. (3) A decrease in NK cell activity, which could be restored with a three months GH therapy, was found in GHD children. The IL 1α and IL 2 activities in GHD children were decreased and showed a tendency to increase with GH supplement. Conclusion The above data supported the findings of earlier reports that some relationship existed between GH and the immune system.
出处
《中华儿科杂志》
CAS
CSCD
北大核心
1998年第12期744-746,共3页
Chinese Journal of Pediatrics
