摘要
合成了一种新型的具有良好生物活性的噻吩并嘧啶酮有机化合物2-叔丁氨基噻吩并[2,3-d]嘧啶-4(3H)酮(SDA),通过核磁共振、红外光谱及元素分析等对其结构进行了表征;应用荧光光谱及紫外可见光谱法研究了该化合物(SDA)与牛血清白蛋白(BSA)之间在不同温度下的相互作用.实验表明,SDA能强烈猝灭BSA的内源荧光,猝灭机理为动态猝灭.在此基础上计算了二者相互作用的结合常数、结合位点数及热力学参数等.结果表明,SDA与BSA分子以物质量的比1:1结合,其结合反应主要是熵驱动,主要作用力是疏水力.同时,应用同步荧光考察了SDA对BSA构象的影响.
a new compound 2-tert butyl amine-thieno [- 2, 3-d] pyrimidin-4 （ 3H）-ones （SDA） have been synthesized and characterized by 1H NMR,IR and elemental analysis. The interaction between 2-tert butyl amine-thieno [2,3-d] pyrimidin-4（3H）-ones（SDA） and Bovine Serum Albumin was investigated by fluorescence and absorption spectroscopy. The experimental results showed that the fluorescence quenching of BSA by SDA was a result of the formation of The SDA-BSA complex and dynamic quenching occurred in the fluorescence quenching. The binding sites number n, apparent corporation constant K and corresponding thermodynamic parameters AH, AG, AS were calculated. The research results indicated that SDA bound BSA with molar ratio of 1 ： 1 and the binding reaction was mainly entropy-driven and hydrophobic interactions forces played major role in the reaction. The effect of SDA on the conformation of BSA was analyzed using synchronous fluorescence spectrocopy.
出处
《华中师范大学学报（自然科学版）》
CAS
CSCD
北大核心
2010年第1期70-75,共6页
Journal of Central China Normal University：Natural Sciences
基金
湖北省教育厅重点项目(D20092804)
作者简介
E-mail：liyan750606@yahoo．com.cn.
