摘要
目的研究胃癌及癌旁组织中促凋亡基因 Bax 的表达状态及其与细胞凋亡之间的关系。方法应用免疫组织化学方法及脱氧核糖核酸末端转移酶介导的缺口末端标记(TUNEL)技术对50例胃癌及33例癌旁组织中 Bax 表达及凋亡细胞的密度和分布进行原位观察和比较。结果癌旁组织中 Bax 蛋白的表达率、表达强度显著高于胃癌组织(P<0.01)。Bax 蛋白在胃癌中的表达与患者性别、年龄、肿瘤大小、浸润深度、淋巴结转移及临床分期差异无显著性(P>0.05),与胃癌形态学分型及分化程度有关(P<0.05及 P<0.01)。癌旁组织中的凋亡细胞密度显著高于胃癌组织(P<0.01)。Bax 蛋白表达阳性胃癌及癌旁组织中的凋亡细胞指数明显高于 Bax 阴性组(P<0.01)。结论 Bax 蛋白对胃癌及癌旁组织中的细胞凋亡具有促进性调控作用。在胃粘膜细胞恶性转化过程中可能存在细胞凋亡受抑,而 Bax 蛋白表达减少可能是细胞凋亡受抑的机制之一。细胞凋亡调控异常在胃癌发病中可能起重要作用。
Objective To investigate the expression of proapoptotic gene Bax and its association with apoptosis in gastric carcinoma and paracancerous tissues. Method Bax protein expression and the density and distribution of the apoptotic cells in situ in 50 gastric carcinoma and 33 paracancerous tissues were observed using immunohistochemical staining and terminal deoxynucleotidyl transferase mediated nick end labelling(TUNEL)technique.Result The positive rate and staining intensity of Bax protein in paracancerous tissues were significantly higher than that in gastric carcinoma (P<0.01).In gastric carcinoma,the rate of Bax protein expression had no correlation with the age and sex of patients,tumor size, lymph node metastasis,the depth of invasion and Dukes' staging of tumors (P>0.05 for each),it was strongly associated with intestinal type gastric carcinoma and well/moderately-differentiated gastric carcinoma (P<0.05 and P<0.01).The density of apoptotic cells in paracancerous tissues was significantly higher than that in gastric carcinoma (P<0.01).In gastric carcinoma and paracancerous tissues,the density of apoptotic cells in the Bax positive group was higher than that in the Bax negative group (P<0.01).Conclusion The Bax protein can promote apoptosis in gastric carcinoma and paracancerous tissues.Proapoptotic gene Bax may take part in the regulation of apoptosis in the process of gastric carcinogenesis.The abnormal regulation of apoptosis may play an important role in the pathogenesis of gastric carcinoma.
出处
《中华消化杂志》
CAS
CSCD
北大核心
1998年第6期325-327,共3页
Chinese Journal of Digestion
