摘要
目的:研究黄芩苷对光滑念珠菌、近平滑念珠菌、克柔念珠菌、季也蒙念珠菌等非白念珠菌生物膜的影响。方法:96孔微量培养板上构建4种非白念珠菌生物膜;微量稀释法检测黄芩苷对4种非白念珠菌最低抑菌浓度(MIC);XTT减低法评价黄芩苷对4种非白念珠菌生物膜SMIC及菌细胞黏附性的影响。结果:4种非白念珠菌在96孔板中可形成成熟的生物膜;黄芩苷对4种非白念珠菌MIC分别是125,250,125,62.5 mg.L-1;黄芩苷对4种非白念珠菌生物膜的SMIC50分别是(1 000,500,125,250 mg.L-1;SMIC80均大于或等于1 000 mg.L-1。黄芩苷对4种非白念珠菌的黏附有一定的抑制效应。结论:黄芩苷对近平滑念珠菌、克柔念珠菌、季也蒙念珠菌生物膜有较强的抑制作用。
Objective ： To investigate the effects of baicalin against Candida glabrata, C. parapsilosis, C. krusei and C. guilli- ermondii biofihns. Method： 96-well microtitre plates were used to set up the biofilms ; microdilution method was applied to detect minimal inhibitory concentration（MIC） of baicalin for the four non-albians Candida, and XTT reduction assay was adopted to determine sessile minimal inhibitory concentration（ SMIC ） of baicalin against the four isolates and to detect the effects on adhesion of the fungal cells. Result： MICs of baicalin for the four non-albians Candida cells were 125,250, 125, 62. 5 mg· L^-1 , respectively. The four nonalbians Candida could form mature biofihns on 96-well microtitre plates. SMIC50 of baicalin for the four isolates were 〉 1 000, 500, 125, 250 mg · L^-1 , respectively. SMIC80 for the four isolates were greater than br equal to 1 000 mg· L^-1. Baicalin showed potent inhibitory effects on adhesion of the four non-albians Candida cells. Conclusion： Baicalin displays substantial inhibitory effects on C. parapsilosis, C. krusei and C. guilliermondii biofilm.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2010年第5期639-641,共3页
China Journal of Chinese Materia Medica
基金
安徽省自然科学基金项目(090413092)
安徽省高校自然科学研究项目(KJ2008B223)
作者简介
[通信作者]汪长中，副教授，在读博士生，主要从事细菌生物膜研究，Tel：13956096007，E-mail：ahwcz63@sina．com.
