摘要
目的探讨白细胞介素-17A,-17F(IL-17A,-17F)基因多态性与慢性萎缩性胃炎(chronic atrophic gastritis,CAG)的相关性。方法采用病例-对照研究方法,收集经组织学确诊的慢性萎缩性胃炎患者129例和健康对照人群223例,收集外周血标本,提取DNA,运用TaqManMGB探针方法进行多态性检测。同时所有样本经组织快速尿素酶检测(RUT)和血清酶联免疫吸附法(ELISA)抗Hp-IgG抗体检测,以测定人群幽门螺杆菌(Helicobater pylori,Hp)感染率。结果IL-17A(rs2275913,G-197A)、IL-17F(rs76378,7488T/C)位点的基因型和等位分布在慢性萎缩性胃炎组和健康献血员正常对照组中差异均无统计学意义(P>0.05)。IL-17F(rs766748,6400A/G)在慢性萎缩性胃炎组中的等位频率显著高于正常对照组(P=0.004)。与未携带有IL-17F 6400A/GA等位的个体相比,携带有IL-17F 6400A/GA等位的个体显著的增加了慢性萎缩性胃炎的易感性。经非条件Logistic回归校正年龄、性别、Hp感染等因素后,在显性模式下,IL-17F 6400A/GSNP位点与慢性萎缩性胃炎仍显著相关(显性模式:OR=2.271,95%CI=1.259-4.095,P=0.006)。在伴有Hp感染的所有群体中进行比较,IL-17A,-17F各位点基因型未见明显差异。结论IL-17F 6400A/G基因多态性与慢性萎缩性胃炎的发生风险显著相关。
Objective To determine the possible association of the 3 polymorphisms, IL-17A rs2275913 G-197A, IL-17F rs763780 7488T/C and IL-17F rs766748 6400A/G） in the IL-17 gene with the susceptibility to gastroenteric disease caused by H. pylori in Chinese population. Methods A hospital-based case-control study was conducted, and 223 unrelated healthy blood donors and 129 chronic atrophic gastritis （CAG） patients were recruited from Chongqing. Three polymorphisms were genotyped by TaqMan-MGB-PCR a- nalysis. The associations with the susceptibility to CAG disease were estimated by Logistic regression. The infection of H. pylori level were evaluated by the level of serum anti-Hp-IgG antibody by using enzyme-linked im- munosorbent assay （ELISA） and the urease of gastric mucosa detected with rapid urease testing （RUT）. Resuits There was no significant difference in allele frequencies or genotype distributions of IL-17A G-197A and IL-17F 7488T/C between blood donors and CAG patients （P 〉 0.05）. However, the IL-17F 6400A/G allele frequencies were significantly higher in CAG patients than those in blood donors （ P = 0. 004）. Subjects bearing at least one IL-17F 6400A/G A alleles had an increased susceptibility to CAG diseases compared with those without IL-17F 6400A/G A alleles （ Dominant model, P = 0. 006, OR = 2. 271, 95% CI ： 1. 259 - 4. 095）. There was no significant difference in allele frequencies or genotype distributions of three polymorphisms in the IL-17A and IL-17F gene between the all subjective with H. pylori infection or without. Conclusion Our resubs suggest that the genetic variation at the IL-17F locus affects the susceptibility to CAG in Chinese population and emphasizes the importance of IL-17 in the pathophysiology of gastroenteric disease.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2010年第6期525-528,共4页
Journal of Third Military Medical University
基金
国家重点基础研究发展计划(973计划
2009CB522600)~~
作者简介
[通信作者]郭刚，电话：（023）68752315，E-mail：guogang7001@163.com
