摘要
目的观察槲皮素(Que)对大肠癌细胞增殖、侵袭力的影响,并探讨其可能机制。方法加入不同浓度的Que培养大肠癌细胞SW-480后,用软琼脂克隆培养试验检测SW-480的增殖能力,用Boyden小室模型法检测SW-480侵袭力,用荧光实时定量RT-PCR检测SW-480的滋养层细胞表面抗原2(TROP-2)mRNA,以Western blot法检测SW-480的TROP-2蛋白。结果SW-480加入Que培养后,恶性增殖和穿膜细胞数均明显下降,且呈剂量依赖性(P均<0.01);同时其TROP-2 mRNA和蛋白表达水平均明显下调,且呈时间和剂量依赖性(P均<0.01)。结论Que可明显抑制大肠癌SW-480细胞的增殖、侵袭力,其机制可能与下调TROP-2基因表达有关。
Objective To study the effects and mechanism of quereetin on human eoloreetal cancer cell, Methods Human eolorectal cancer cells SW-480 were treated with different doses of quereetin, and the growth of anchorage independence of SW-480 was studied by colony formation in soft agar, the invasiveness of SW-480 was determined by Boyden chamber, the expression of mRNA and protein of trophoblast cell-surface antigen-2 （TROP-2）gene were detected by real time RT-PCR and Western blot assay, respectively. Results Transmembrane cell and anchorage independence growth of querce- tin group decreased in dose-dependent manners（P 〈0.01 ）. The level of mRNA and protein of TROP-2 of SW-480 treated with quercetin reduced in time- and dose-dependent manners（P 〈0.01 ）. Conclusions Quercetin can inhibit invasiveness and proliferation of human colorectal carcinoma cells, and decreasing the expression of TROP-2 gene may play an important role in its mechanism.
出处
《山东医药》
CAS
北大核心
2010年第10期1-2,共2页
Shandong Medical Journal
基金
江苏省自然科学基金资助项目(2009205)
镇江市社会发展基金资助项目(SH2009014)
关键词
大肠肿瘤
槲皮素
细胞增殖
细胞侵袭力
滋养层细胞表面抗原
colorectal carcinoma
quercetin
cell proliferation
cell invasiveness
trophoblast cell-surface antigen
作者简介
通讯作者
