摘要
目的观察红曲对胶原诱导性关节炎（CIA）大鼠血清中趋化因子的影响。方法利用牛Ⅱ型胶原建立Wistar大鼠CIA模型，造模成功后随机分为空白对照组、生理盐水CIA模型对照组、布洛芬阳性药物对照组和红曲大剂量、中剂量及小剂量组并灌胃给药。免疫后第48天处死大鼠，抽取静脉血，取血清。采用酶联免疫吸附试验（ELISA）观察各组对血清中细胞因子肿瘤坏死因子（TNF）-α、白细胞介素（IL）-6和趋化因子IL-8、单核细胞趋化因子（MCP）-1、调节正常T细胞表达和分泌因子（RANTES）和干扰素1诱导蛋白10（IP-10）的影响。结果红曲大、中、小剂量组能显著降低血清中细胞因子TNF-α[（67．4±1．7）pg／ml、（64．3±12．8）pg／ml和（61．2±9．9）pg／ml]、IL-6[（144±21）pg/ml、（133±29）pg/ml和（115±31）pg／ml]的水平，与模型组[分别为（91．4±9．4）pg／ml和（180±14）pg／ml]比较差异有统计学意义（P〈0．05）；红曲大剂量，中剂量可降低IL-8[（62±7）pg/ml和（61±11）pg／ml]的水平，与模型组[（87±18）pg/ml]比较差异有统计学意义（P〈0．05）；红曲各剂量组能显著降低血清中炎性趋化因子MCP-1、RANTES和IP-10的表达，与模型组比较差异有统计学意义（P〈0．05）。结论红曲抗炎作用可能是和抑制炎症因子相关。
Objective To evaluate the effect of red yeast rice on chemokines of collagen-induced arthritis in rats. Methods Rat arthritis was induced by injecting bovine type H collagen （B H C） to Wistar rats. The rats were randomly divided into six groups including the normal control group, the CIA control group which were treated with normal saline, ibuprofen group, large dose red yeast rice group, moderate dose of red yeast rice group and small dose of red yeast rice group. Mice were sacrificed after 48 days of immune injection and their venous blood were collected. The effect of cytokines and chemokines on CIA was measured by ELISA. Results Red yeast rice significantly degraded the expression of TNF-α, IL-6 compared with the model groups, the difference was significantly different （P〈0.05）. The degree of IL-8 decrease was more evident in the high dose of Red yeast rice group and moderate dose of Red yeast rice group than that of the model group （P〈0.05）. Red yeast rice could significantly degrad the expression of MCP-1, RANTES and IP- 10 in serum of CIA rats （P〈0.05）. Conclusion The mechanism of anti-inflammatory action of red yeast rice is related to the inhibition of inflammatory factors.
出处
《中华风湿病学杂志》
CAS
CSCD
北大核心
2010年第2期80-83,共4页
Chinese Journal of Rheumatology
基金
国家自然科学基金青年基金（30600811）
