摘要
为了研究番茄幼苗在缺磷胁迫下根系形态发育与生长素、生长素信号转导途径中的转录因子NAC1,以及调控NAC1表达的上游miR164之间的关系。试验以5和500μmol/L磷浓度作为缺磷胁迫和对照,检测了外源生长素NAA(1-naphthalene acetic acid)及生长素抑制剂NPA(N-1-naphthylphthalamic acid)对侧根形成的影响;同时采用RT-PCR检测了NAC1和miR164在缺磷胁迫下的时序表达。结果表明,缺磷胁迫下侧根大量形成与生长素及其运输密切相关,在侧根迅速形成的24 h内,NAC1的表达在缺磷胁迫下增强;而其上游的miR164表达降低,从而揭示了缺磷胁迫下侧根形成与miR164调节NAC1表达之间的关系。
This study investigated the relationship between the development of tomato lateral roots and auxin,NAC1（transcription factor involved in the auxin signal transduction） and miR164 expression under P deficiency.Effects of exogenous auxin NAA（1-naphthalene acetic acid） and its transport inhibitor NPA（N-1-naphthylphthalamic acid） on the lateral root development were analyzed under P deficiency（5 μmol/L Pi） versus the control（500 μmol/L Pi）.The time course of the expression pattern of miR164 and its target gene,NAC1,were analyzed by RTPCR.The results showed that the development of lateral roots was promoted by NAA and blocked by NPA.During 12 h of lateral root initiation,expression of NAC1 was strongly up-regulated,while miR164 was down-regulated in P-deficient plants.Our data suggest that miR164,together with its target（NAC1）,is involved in the mediation of P-deficiency-induced lateral root development.
出处
《植物营养与肥料学报》
CAS
CSCD
北大核心
2010年第1期166-171,共6页
Journal of Plant Nutrition and Fertilizers
基金
国家自然科学基金项目(30700488)
教育部科学技术研究重点项目(107060)
江苏省普通高校研究生科研创新计划项目资助
作者简介
作者简介：曾后清（1986-），男，江西吉安人，硕士研究生，主要从事缺磷胁迫下植物中miRNA的筛选与鉴定工作。Tel：025—84396393，E-mail：2007103140@njau．edu．cn.
通讯作者Tel：025—84396393，E-mail：yiyongl973@njau．edu．cn
