摘要
为研究血管内皮生长因子(vascular endothelial growth factor,VEGF)对绵羊卵母细胞体外成熟过程中细胞超微结构变化的影响,根据前期研究结果,在绵羊卵母细胞体外成熟培养液(TCM199+BSA)中添加不同剂量的VEGF(对照组0 ng.mL-1和试验组5 ng.mL-1)进行成熟培养。利用透射电镜技术进行分析,研究结果显示:①VEGF加速了微绒毛的发育和变化,促进了微绒毛游离端斜行插入透明带,增加了细胞内外物质交流和养分的吸收;②VEGF对卵母细胞成熟过程中高尔基体的影响不显著;③发现特征性绵羊卵母细胞线粒体———带帽状线粒体,并且VEGF对线粒体的迁移有一定的促进作用;④VEGF的添加对绵羊卵母细胞体外成熟过程中脂滴数量的增加没有显著的作用,但VEGF减少了小脂滴聚集为大脂滴的数量。
Human recombinant VEGF165 was employed at 5 ng·mL^-1 in maturation media（TCM199＋ bovine serum albumin,BSA） to investigate the effect of vascular endothelial growth factor（VEGF） on ultrastructure change of the ovine oocytes maturation in vitro in this study.The results showed that the development and change of microvilli on the surface of oocytes could be affected by VEGF in the maturation media.VEGF could promote the oocytes absorbing nutrient substance and their communication.There was no significant effect of VEGF on the temporal and spatial translocation or change of Golgi in ovine oocytes undergoing in vitro maturation.There were many mitochondria with ＂cap＂ in the oocytes cytoplasm.The development and transfer of mitochondria was effected by VEGF.There were no significant effects of VEGF on the accumulation of cytoplasmic lipid droplet,but VEGF could avoid the integration of many droplets into bigger ones.
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2010年第2期167-173,共7页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
国家自然科学基金(30371035
30771551)
关键词
血管内皮生长因子
绵羊卵母细胞
体外成熟
超微结构
vascular endothelial growth factor
ovine oocyte
maturation in vitro
ultrastructure
作者简介
曹忻（1978-），女，宁夏人，讲师，博士，主要从事动物遗传育种与繁殖方面的研究工作，E—mail：caoxinjuliet@163．com
通讯作者：罗海玲，E-mail：luohailing@call．edu．cn
