摘要
目的:检测动脉粥样硬化斑块中是否存在牙周致病菌。方法:共选取在手术过程中剥离的、已有动脉粥样硬化改变的动脉内膜标本10例,采用PCR扩增16S rDNA(16S ribosomal DNA)片段的方法,检测7种牙周可疑致病菌,包括牙龈卟啉单胞菌(Porphyromonas gingivalis,Pg)、福赛坦菌(Tannerella forsythia,Tf)、伴放线聚生杆菌(Aggregatibacter actinomycetemcomitans,Aa)、中间普氏菌(Prevotella intermedia,Pi)、变黑普氏菌(Prevotella nigrescen,Pn)、牙密螺旋体(Treponema denticola,Td)及直肠弯曲菌(Campylobacter rectus,Cr)。结果:10例标本中有3例检测出了牙周可疑致病菌,3例样本均检出了Pg,这3例中有1例同时检出了Tf,另1例同时检出了Pn;其余7例标本未检出相关牙周致病菌。结论:动脉粥样硬化斑块样本中检出了部分牙周可疑致病菌,为牙周炎与动脉粥样硬化之间可能存在联系提供了初步证据。
Objective：To detect periodontopathic bacterial DNA in atherosclerotic vascular lesions in a group of Chinese patients by using polymerase chain reaction（PCR） techniques.Methods： Ten human specimens of atherosclerotic plaque were obtained sterilely.The sclerotic lesions were blade into fine pieces and DNA was isolated from the samples.To detect Porphyromonas gingivalis（Pg）,Tannerella forsythia（Tf）,Aggregatibacter actinomycetemcomitans（Aa）,Prevotella intermedia（Pi）,Prevotella nigre-scens（Pn）,Treponema denticola（Td）,Campylobacter rectus（Cr）,PCR amplification of bacterial 16S ribosomal DNA（rDNA） was carried out.Results： PCR assays for bacterial 16S rDNA indicated the presence of periodontal pathogens in 3 out of 10 surgical specimens.DNA of Pg were found in 3 samples,Tf was found only in one among the 3 samples and Pn was found in another sample among the 3 samples.Conclusion： The data confirm that DNA of periodontal pathogens present in atherosclerotic plaques.Pg,Tf,Pn may play a role in the development and progression of atherosclerosis in these Chinese patients.Further studies with large size samples are needed.
出处
《北京大学学报（医学版）》
CAS
CSCD
北大核心
2010年第1期33-36,共4页
Journal of Peking University:Health Sciences
基金
国家自然科学基金(30471883)资助~~
关键词
牙周炎
动脉粥样硬化
紫单胞菌
龈
DNA
细菌
Periodontitis
Atherosclerosis
Porphyromonas gingivalis
DNA
bacterial
作者简介
Corresponding author＇s e—mail：kqouyangxy@bjmu．edu．cn
