摘要
以盆栽小白菜(四月蔓)为试材,研究涝渍及恢复期间小白菜表观伤害及活性氧代谢的变化。试验结果表明,涝渍后小白菜植株黄化严重,涝渍4d,8d和12d(恢复4d)后植株死亡率分别为20%,50%和80%;涝渍后叶片叶绿素含量呈直线下降趋势,恢复4d略有回升;涝渍使小白菜叶片细胞膜透性逐渐增强;涝渍后造成活性氧代谢平衡体系破坏,涝渍期间叶片愈创木酚-过氧化物酶(G-POD)活性持续升高,恢复期间有所下降;受涝后超氧化物歧化酶(SOD)活性先升后降,恢复期间逐渐升高;受涝后抗坏血酸过氧化物酶(AsA-POD)持续升高,恢复期间缓慢下降;受涝及恢复期间叶片的丙二醛(MDA)和脯氨酸(PRO)含量变化同AsA-POD一致。总之,涝渍造成小白菜活性氧代谢失常,引起小白菜产量显著降低。
Using pakchoi （Siyueman） as material, we observed plant visible injuries under waterlogging stress for 4, 8 d and physiological influence after recovery 4 d The results showed that plant etiolate seriously after waterlogging, and root decayed to death （ death rates were about 20%, 50% and 80% after plant waterlogged 4, 8 d and recovered 4 d ）. Leaf chlorophyll content showed descending trend, but gently increased at 4th day after recovery. Waterlogging caused plants membrane permeability gradually increasing. Waterlogging destroyed the balance of Chinese cabbage cell protective sys- tems. After waterlogging, guaiacol peroxidase （G-POD） and superoxide dismutase （SOD） activities gently increased at 4th day, then G-POD activity rapidly increased at 8th day, but SOD activity decreased at 8th day. During the period of recovely, G-POD activity gently decreased, then SOD activity gradually increased. With waterlogging degree increasing, leaves aseorbic acid peroxidase （AsA-POD） activities gently increased at 4th day after waterlogging, then rapidly in- creased at 8th day after waterlogging, but decreased during the period of recovery. Malonaldehyde （MDA） activities in- creased at 4th day after waterlogging, then rapidly increased at gth day after waterlogging, but decreased during the period of recovery. Proline （PRO） content gently increased at 4th day after waterlogging, then rapidly increased at 8th day after waterlogging, but decreased during the period of recovery.
出处
《长江蔬菜》
2009年第12X期35-38,共4页
Journal of Changjiang Vegetables
基金
长江大学博士启动基金(2007018)
关键词
小白菜
涝渍
活性氧代谢
Pakchoi
Waterlogging stress
Active oxygen metabolism
作者简介
李德明（1972-），男，副教授，浙江大学博士／博士后，主要研究方向为植物矿质营养、逆境生理生化及发育生理和园林植物应用，电话：13207295391。E—mail：lidmn@163．com
