摘要
应用病毒感染的鸡胚材料免疫新西兰兔的方法制备抗鸡传染性支气管炎病毒(IBV)单因子血清,然后在鸡胚气管环培养(Tracheal organ cultures,TOC)上对广西分离的7个IBV代表性毒株和3个常用疫苗株进行交叉病毒中和试验。结果显示,10个毒株被分为6个血清型。根据试验所得的R值,应用聚类分析法分析了各血清型毒株之间的亲缘关系,显示目前在广西流行的IBV野毒株之间以及其与疫苗株间的抗原性存在很大程度的差异,分属不同的血清型。同时还对IBV基因分型和血清分型之间的关系进行了探讨。
Monovalent antisera against seven field isolates and three commonly used vaccine strains of infectious bronchitis virus （IBV） were prepared in rabbits and were used in the virus neutralization test against the heterogeneous and homogeneous viruses on tracheal organ cultures （TOC） of chicken embryo. Relatedness values of each virus calculated from cross neutralization antibody titer showed that 6 serotypes were distinguished among the 10 viruses tested. Phylogenetic analysis of the different serotypes of viruses also indicated that there was a divergence among the field isolates and between the isolates and the vaccine strains, belonging to different serotypes. The data of the study provides a reliable basis for catching up the emerged antigenic variation of the field viruses and developing more effective vaccine for the disease control in Guangxi. The relationship between genotyping and serotyping was also discussed.
出处
《中国兽医学报》
CAS
CSCD
北大核心
2010年第2期183-187,共5页
Chinese Journal of Veterinary Science
基金
国家自然科学基金资助项目(30700599)
广西自然科学基金资助项目(桂科青0728003)
广西区教育厅科研资助项目(C150002)
广西科技攻关资助项目(0993009-2)
关键词
鸡传染性支气管炎病毒
气管环组织培养
交叉中和试验
血清型
抗原变异
聚类分析
infectious bronchitis virus
tracheal organ cultures
cross neutralization test
serotype
antigenic variation
phylogenetie analysis
作者简介
王秀英（1983-），女，硕士。
通讯作者，E-mail：pingwei8@126．com
