摘要
[目的]提取"顽拗"植物春石斛的基因组DNA,并对其进行质量检测。[方法]采用普通CTAB法、试剂盒法和改良CTAB法提取10个春石斛品种的基因组DNA,然后分别用紫外分光光度计法、琼脂糖凝胶电泳法和荧光AFLP法对3种方法所提取的基因组DNA质量进行检验。[结果]改良CTAB法所提取春石斛基因组DNA得率较高,基本排除了春石斛体内多糖等次生代谢物质对基因组DNA质量的干扰,完全能满足AFLP等分子生物学试验对DNA质量的要求;其他2种方法所提取的基因组DNA则不能满足试验要求。[结论]改良CTAB法为较好的适合春石斛基因组DNA提取的方法。
[ Objective ] The research aimed to extract the genomic DNA from Spring Dendrobium, and examine its quality. [ Method] Three different methods, CTAB method, kit method and modified CTAB method, were chose to extract the high quality DNA from the recalcitrant spring Dendrobium. Then UV speetrophotometer method, ngarose gel electrophoresis method and fluorescent AFLP method were used to examine the quality of genomie DNA extracted by 3 extraction methods. [ Result ] The ratios of the genomic DNA extracted by the modified CTAB method were higher, and could meet the require of molecular biological experiment. But the genomic DNA extracted by the other two methods could not meet the require. [ Conclusion] The modified CTAB method is good and suitable for extracting the genomic DNA from Spring Dendrobium.
出处
《安徽农业科学》
CAS
北大核心
2010年第3期1156-1159,1166,共5页
Journal of Anhui Agricultural Sciences
基金
浙江省科技厅重点科研项目(2005C22082)
关键词
春石斛
顽拗植物
基因组DNA
提取
改良CTAB法
Spring Dendrbium
Recalcitrant plant
Genomic DNA
Extraction
Modified CTAB method
作者简介
郑泉（1969-），男，河南商丘人，在读博士，高级工程师，从事观赏植物生物技术研究。
通讯作者，E-mail：guowm2008@grnail．com。
