摘要
目的:观察红花注射液对内毒素性急性肝损伤大鼠的干预作用及可能的机制。方法:将清洁级雄性SD大鼠80只随机分为模型组、红花注射液干预组(红花组)、还原型谷胱甘肽干预组(TAD组)及空白对照组,模型组时相点设为6h、24h、48h,红花注射液干预组、还原型谷胱甘肽干预组(TAD组)时相点同模型组;每时相点10只动物;空白对照组10只动物。模型组以腹腔注射内毒素脂多糖(LPS)+D-氨基半乳糖胺(D-GalN)诱导建立大鼠急性肝损伤模型;红花组、TAD组于建模前0.5h分别以红花注射液、还原型谷胱甘肽尾静脉注射;模型组、红花组、TAD组分别于建模后6h、24h、48h及空白对照组于6h时取腹腔静脉血和肝组织,检测血清丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST),苏木素-伊红(HE)染色观察肝组织病理学变化,免疫组织化学法检测肝组织Bax、Bcl-2蛋白表达并计算其比值(Bax/Bcl-2)。结果:与空白对照组比较,模型组各时间点大鼠血清ALT、AST水平显著升高(均P<0.01);肝组织Bax、Bcl-2表达及其比值明显上升(均P<0.01)。与模型组相比,红花组、TAD组各时间点大鼠肝组织病理损伤程度明显改善;血清ALT、AST水平不同程度地下降(P<0.05或P<0.01);肝组织Bax、Bcl-2表达及Bax/Bcl-2下降(均P<0.01)。红花组与TAD组间比较差异均无统计学意义(P>0.05)。结论:红花注射液对内毒素性急性肝损伤有较好的保护作用,其机制可能与下调肝组织Bax、Bcl-2表达及其比值,抑制肝组织细胞过度凋亡相关。
Objective：To observe the protective effects and mechanism of safflower injection （SI） on rats with acute hepatic injury （AHI） induced by endotoxin. Methods： 80 male Sprague - Dawley rats were randomly divided into ten groups, three SI treated groups, three reduced glutathione treated groups, three AHI model groups, and one control group. The rats were intraperitoneal - injected with Lipopolysaccharide （LPS） plus D - galactosamine （ D - GaIN ） for modeling. The SI treated groups was injected SI into a tail vein, and the reduced glutathione treated groups was was injected reduced glutathione as the positive control. The blood from the abdominal vein and liver tissue samples were obtained at 6h, 24h and 48h after modles established. The contents of alanine aminotransferase （ALT） and aspartate aminotransferase （AST） were tested, the hepatic pathological changes were observed with hematoxylin -eosin （HE） stain, and the expression of Bax, Bcl - 2 were detected by immunohistochemical studies. Results ： Compared with the control group, sermn levels of ALT and AST in rats of the model groups significantly elevats at 6h, 24h and 48h time points （P 〈 0.01 ） ; and the expression of Bax, J3cl - 2 protein rise at every time points （ P 〈 0. 01 ） , the ratio of Bax/Bcl - 2 obvi- ously inrise （P 〈 0.01 ）, Compared with the model groups, treatment with SI or reduced glutathione could obviously improve the pathological changes, reduce serum levels of ALT and AST （ P 〈 0.05 or P 〈 0.01 ）, significandy decrease the expression of Bax, Bc1-2 protein and reduced Bax/Bc1-2 （P 〈0.01） at 6h, 24h and 48h. There was no significant deviation between groups treated with SI and reduced glutathione. Conclusion It is shown that SI has promising protective effects on rats with AHI caused by LPS plus D -GalN, and its mechanism is probably related to the inhibition of liver tissue cell apoptosis, reducing liver tissue Bax, Bcl -2 protein expression and Bax/Bcl- 2.
出处
《中华中医药学刊》
CAS
2010年第1期159-162,共4页
Chinese Archives of Traditional Chinese Medicine
基金
浙江省中医药科技项目(2007ZA009)
作者简介
作者简介：陈英（1983-），女，浙江临海人，硕士研究生，研究方向：中西医结合临床（内科）急诊医学。
通讯作者：黄小民（1954-），男，浙江杭州人，主任医师，研究方向：中西医结合临床急诊医学。
