摘要
目的观察经环氧乙烷灭菌处理的物品感染生物治疗细胞后对其的致畸性。方法将分离诱导的3种细胞分别接种至环氧乙烷灭菌处理保存的不同时间及高温高压灭菌的玻璃培养瓶中进行培养,分别将脐血诱导的NK/CIK细胞、人骨髓间充质干细胞和脐血CD34+细胞,接种至环氧乙烷灭菌保存至培养瓶中,分别为1 d(A组)、3个月(B组)、>6个月(C组);接种至高温高压蒸汽灭菌的玻璃培养瓶中D组为对照组;培养10 d,观察细胞的生长状况、形态、染色体断裂、断片、裂隙及核内染色体复制情况,计算染色体畸变率。结果A、B组的NK/CIK细胞的诱导率明显低于C、D两组,差异有统计学意义(P<0.05),染色体断裂、断片及裂隙数量均明显高于C、D两组,差异有统计学意义(P<0.05),而C组与D组比较差异无统计学意义(P>0.05)。结论经环氧乙烷灭菌处理保存至1 d和3个月的物品对细胞产生明显的损伤作用,>6个月的物品对细胞未造成明显损伤作用;环氧乙烷对细胞会产生致畸致突变作用,建议采用保存至>6个月的灭菌物品,从而保证生物治疗的安全有效实施。
OBJECTIVE To observe the teratogenecity of ethylene oxide sterilized materials to cells which are used for biotherapy.METHODS Three kinds of cells were cultivated in ethylene oxide sterilized culture flasks which were conserved for different time and high temperature/high pressure sterilized vitric culture flasks.NK/CIK cells induced from cord blood,human mesenchymal stem cell and CD34＋ cells were cultivated in ethylene oxide sterilized culture flasks which were conserved for one day（group A）,for three months（group B）,for more than six months（group C） and cultivated in high temperature/high pressure sterilized vitric culture flasks（group D）.After ten days,not only the growth and morphology of cells but also the breakages fragmentations and gaps in chromosomes and replication of chromosome in nucleus were observed.According to these data,the malformation rate was calculated.RESULTS Compared with groups C and D,the inductivity of groups A and B obviously degraded（P〈0.05）.The breakages,fragmentations and gaps in chromosomes of groups A and B were more than those of groups C and D（P〈0.05）.There were no differences betweens group C and D（P〉0.05）.CONCLUSIONS Compared with the control group,the materials which were sterilized by ethylene oxide and conserved for less than one day and three months can bring damage to cells.However,the damage is not discovered in the materials which were conserved for more than six months.Ethylene oxide can induce cell aberrant and mutation.Therefore,it is suggests that materials be conserved for more than six months to ensure safe and effective enforcement of biotherapy.
出处
《中华医院感染学杂志》
CAS
CSCD
北大核心
2009年第24期3365-3367,共3页
Chinese Journal of Nosocomiology
基金
广东省科技计划项目(2008B030301066)
广东省自然科学基金(7001603)
关键词
环氧乙烷
间充质干细胞
脐血
Ethylene oxide
Mesenchymal stem cell
Cord blood
