摘要
对毛细管电泳(CE)测定畜禽肌肉中肌红蛋白和血红蛋白含量方法的建立进行研究。采用P/ACETMMDQ高效毛细管电泳仪,电泳缓冲液为50mmol/L柠檬酸-20mmol/L柠檬酸三钠,pH2.6,电泳温度25℃,电压22kV,紫外检测波长196nm。结果表明:肌肉中肌红蛋白和血红蛋白含量测定结果相对标准偏差为0.69%和0.90%,后加标准品和前加标准品回收率分别为93.26%～97.84%、90.32%～97.46%和79.89%～84.14%、79.52%～83.65%。该方法简单、快速、对同时测定畜禽肌肉色素物质肌红蛋白和血红蛋白含量准确度良好,是评价肉品质参数的一种值得推广应用的新方法。
The aim of this research is to develop a method of CE assay of myoglobin and hemoglobin in animal muscles. The P/ACETM MDQ high performance capillary electrophoresis was applied in the experiment. The electrophoresis buffer system was comprised of 50 mmol/L C6H8O7·H2O and 20 mmol/L Na3C6H5O7·2H2O with pH 2.6. The electrophoresis temperature was 25 ℃ and the voltage was 22 kV. UV detection was monitored at 196 nm. The results showed that the relative standard deviation of myoglobin and hemoglobin in animal muscle was 0.69% and 0.90% and the recoveries of retro-plus standard preparation and pro-plus standard preparation were 93.26%-97.84%, 90.32%-97.46% and 79.89%-84.14%, 79.52%-83.65%, respectively. This method proved the benefits of convenience, short-time and accuracy and will be a new method for assaying the myoglobin and hemoglobin of muscle flavor substance.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2009年第24期340-343,共4页
Food Science
作者简介
蔡海莹（1961-），女，副教授，硕士，研究方向为动物营养与畜产品品质。E—mail：haiying20@ahau．edu．cn
