摘要
目的比较骨髓单个核细胞(MNCs)和骨髓源内皮祖细胞(EPCs)移植促进血流重建的效果,探讨非内皮祖细胞在血流重建中的作用。方法获取Lewis大鼠骨髓MNCs,部分MNCs在体外诱导分化为EPCs。采用Lewis大鼠建立单侧后肢缺血模型。建模后3 d,将模型鼠随机分为3组:(1)对照组(n=6),将0.8 mL D-Hank′s液注入对照组大鼠缺血侧后肢;(2)MNC组(n=6),将8×106个骨髓MNC植入MNC组大鼠缺血侧后肢;(3)EPC组(n=6),将体外培养的8×106个EPC植入EPC组大鼠缺血侧后肢。细胞移植后3周行大鼠后肢动脉造影,检测缺血侧后肢侧支血管数;切取缺血侧后肢腓肠肌,分别行CD31和α-SMA免疫组化染色,计算毛细血管密度和小动脉密度。结果MNC组毛细血管密度与EPC组差异无统计学意义[(31.67±7.87)个/HP vs.(32.83±5.38)个/HP,P>0.05],而均高于对照组(19.67±4.80个/HP)(P<0.05);MNC组侧支血管数与EPC组差异无统计学意义[(4.17±0.75)个vs.(4.50±1.38)个,P>0.05],但均高于对照组[(2.50±1.5)个](P<0.05);MNC组小动脉密度与EPC组差异无统计学意义[(4.83±1.47)个vs.(5.50±2.35)个,P>0.05],亦均高于对照组[(2.17±0.98)个](P<0.05)。结论在骨髓干细胞移植治疗肢体缺血性疾病中,非内皮祖细胞在血流重建中所起的作用与EPC相似。
Objective To compare the effect on revascularization of bone marrow mononuclear cells（BM/MNCs）and endothelial progenitor cells（EPCs） transplantation,and investigate the roles of non-endothelial progenitor cells in angiogenesis.Methods BM/MNCs were harvested from Lewis rats bone marrow.The EPCs were obtained after BM/MNCs were cultured by EBM-2 medium for seven days.Lower limb ischemic model was made on Lewis rats.After limb ischemic model were made,0.8ml D-Hanks,8×10^6 BM/MNCs and 8×10^6 EPCs were injected into control group（n=6）,BM/MNCs group（n=6） and EPCs group（n=6） respectively.At 3 weeks after cells transplantation,the quantity of collateral vascularization was observed with digital subtraction angiography（DSA）.Meanwhile,CD31and α-SMA immunohistochemical stain were used to count the capillary and arteriolar density of the excised gastrocnemius muscle of the ischemic limb.Results There was no significant difference between BM/MNCs group and EPCs group in the density of the capillaries[（31.67±7.87）/HP vs（32.83±5.38）/HP,P〉0.05],the number of collaterals [（4.17±0.75）vs（4.50±1.38）,P〉0.05）] and density of arterioles [（4.83±1.47）vs（5.50±2.35）,P〉0.05）],which were higher than those in control group,respectively.Conclusions Non-endothelial progenitor cells in angiogenesis has the same effects as EPC in the treatment of stem cells transplantation for ischemic disease of the limbs.
出处
《中国普通外科杂志》
CAS
CSCD
北大核心
2009年第12期1259-1262,共4页
China Journal of General Surgery
基金
国家高技术研究发展计划(863)基金资助项目(2006AA02A134)
北京市自然科学基金资助项目(7072031)
北京市委组织部优秀人才基金资助项目(20061D0501800265)
关键词
骨髓单个核细胞
内皮祖细胞
细胞移植
血流重建
Bone Marrow Mononuclear Cells
Endothelial Progenitor Cells
Cell Transplantation
Revascularization
作者简介
佟铸，男，首都医科大学宣武医院主治医师，主要从事血管外科临床和基础方面的研究。
通讯作者：张建 E-mail：tongzhu009@163．com
