摘要
目的:通过短期小剂量双氯酚酸口服制备大鼠NSAID相关小肠损伤模型,探讨不同药物干预对大鼠NSAID相关小肠损伤的影响.方法:48只♂SD大鼠随机分为空白组、实验对照组、药物干预组,其中药物干预组又分为替普瑞酮组、埃索美拉唑组、吉法酯组及甲硝唑组,每组8只.采用双氯芬酸0.78mg/(kg?d),共5d灌胃制备大鼠小肠损伤模型;替普瑞酮、埃索美拉唑、吉法酯、甲硝唑组分别以15.63、4.17、31.25、156.25mg/(kg?d),于造模前1d起灌胃,每天1次,共6d.实验期间大鼠正常饮食饮水.5d后处死所有大鼠,观察小肠黏膜的大体及组织学损伤情况.结果:实验对照组大鼠大体评分及组织学评分均较空白组明显升高(4.38±1.41,4.00±1.85vs0,均P<0.05);与实验对照组比较,替普瑞酮、埃索美拉唑、吉法酯、甲硝唑组大体评分均明显降低(2.38±1.69、2.00±2.27、1.13±1.36、2.00±1.60vs4.38±1.41,均P<0.05),组织学评分除替普瑞酮组外,其余各组均有显著下降(2.25±1.39、1.88±1.25、1.75±0.71、2.00±1.07vs4.00±1.85,均P<0.05);各药物干预组间比较,差异无统计学意义(均P>0.05).结论:短期小剂量双氯芬酸口服即可致小肠黏膜损伤,替普瑞酮、埃索美拉唑、吉法酯、甲硝唑对大鼠NSAID相关小肠损伤具有保护作用,可有效减轻大鼠小肠损伤.
AIM： To compare the preventive effects of different drugs on diclofenac sodium-induced intestinal injury in rats. METHODS： Forty-eight male Sprague-Dawley rats were randomly and equally divided into six groups： normal control group, receiving distilled water 1 mL/（100 g·d） for 5 days; model control group, receiving diclofenac sodium 7.8 mg/（kg·d） for 5 days; and five treatment groups, receiving teprenone 15.63 mg/（kg·d）, esomeprazole 4.17 mg/（kg·d）, gefarnate 31.25 mg/（kg·d） and metronidazole 156.25 mg/（kg·d） for six days, respectively, beginning from the day before dclofenac sodium was given as the model control group. Intestinal mucosal samples were taken 24 hours after the last drug administration to observe and compare morphological and histological changes.RESULTS： Compared with the normal control group, the morphological and histological scores increased significantly in the model control group （4.38 ± 1.41 and 4.00 ± 1.85 vs 0, respectively; both P 〈 0.05）. The morphological scores were much lower in all treatment groups than in the model control group （2.38 ± 1.69, 2.00 ± 2.27, 1.13 ± 1.36 and 2.00 ± 1.60 vs 4.38 ± 1.41, respectively; all P 〈 0.05）. The histological scores were also significantly lower in the esomeprazole treatment group, gefarnate treatment group and metronidazole treatment group than in the model control group （1.88 ± 1.25, 1.75 ± 0.71 and 2.00 ± 1.07 vs 4.00 ± 1.85, respectively; all P 〈 0.05）. There was no signif icant differences in the morphological and histological scores among each treatment group （all P 〉 0.05）. CONCLUSION： Teprenone, esomeprazole, gefarnate and metronidazole can exert protective effects on intestinal injury induced by nonsteroidal anti-inflammatory drugs in rats.
出处
《世界华人消化杂志》
CAS
北大核心
2009年第31期3244-3248,共5页
World Chinese Journal of Digestology
关键词
不同药物
小肠损伤
非甾体抗炎药
Different drugs
Intestinal damage
Non-steroidal anti-inflammatory drug
作者简介
通讯作者：孟立娜．教授，主任医师，310006，浙江省杭州市邮电路549，浙江中医药大学附属第一医院消化科.mln6713@163.com电话：0571—86620283
