摘要
目的：研究氯化锰对生精细胞半胱氨酸蛋白酶3（caspase-3）表达的影响，探讨锰诱发大鼠生精细胞caspase-3表达的机制及锌的拮抗作用。方法：雄性SD大鼠随机分为空白对照组，ZnCl2对照组，15mg／kg和30mg／kgMnCl2组；15mg／kg和30mg／kgMnCl2＋ZnCl2组。免疫组织化学法检测睾丸caspasc-3表达。结果：各染锰组，染锰补锌组及染锰剂量相同的6周组，染锰时间相同的30mg／kgMnCl2组caspase-3阳性细胞率均显著升高，染锰补锌组显著降低。结论：染锰15mg／kg4周可诱发大鼠生精细胞caspase-3表达，且随染锰时间和剂量的增加而增加，两者存在一定时间-效应和剂量-效应关系；摄入含锌100mg／kg的食物可拮抗锰诱发的生精细胞caspase-3表达。
Objective： To study the effects of manganese on the expression of caspase-3 in spermatogenic cells of male rat, and to discuss its mechanism and the antagonize effects of zinc. Methods ： Eighty healthy male rats were randomly divided into blank control, ZnCl2 control, 15 mg/kg MnCl2, 30 mg/kg MnCl2 and 15 mg/kg MnCl2 ＋ZnCl2, and 30 mg/kg MnCl2 ＋ ZnCl2 groups. In the 4^th and 6^th weekend, their testis were collected and the expression of caspase-3 in testis was investigated by immunohistochemical methods （SABC）. Results： The caspase-3-positive-cell rate was increased in every manganese and Mn＋Zn groups, and was decreased in Mn＋Zn groups compared with MnCl2 groups. It increased more in 6 weeks group than that in 4 weeks group, and more in 30 mg/kg than that in 15 mg/kg groups. Conclusion： Lintraperitoneal injection of 15 mg/kg MnCl2 for 4 weeks could induce the expression of caspase-3 in rat spermatogenic cells that exhibited in dose and time dependent. Oral administration of 100 mg/kg zinc suppresses the over expression of caspase-3 in rat spermatogenic cells induced by manganese.
出处
《解剖学杂志》
CAS
CSCD
北大核心
2009年第6期728-730,751,共4页
Chinese Journal of Anatomy
基金
遵义市科技项目基金[遵市科合社字（200707号）]
遵义市红花岗区科技项目基金[遵红科合社字（200622号）]
关键词
锰
锌
生精细胞
半胱氨酸蛋白酶3
manganese
zinc
spermatogenic cell
cysteinyl aspartate-specific protease-3
作者简介
E-mail：hnxxgh6711@163．com
通讯作者，E-mail：lianxiucai@163．com
