摘要
目的:了解子宫内膜癌中环氧化酶-2(cyclooxygenase-2,COX-2)在mRNA与蛋白水平的表达及其临床意义,探讨其与血管生成、细胞凋亡及激素受体的关系。方法:收集41例正常子宫内膜组织及52例子宫内膜癌组织,总结相关的临床资料。采用RT-PCR的方法研究COX-2 mRNA水平的表达。用免疫组织化学方法研究COX-2、血管内皮生长因子(vascular endothelial growth factor,VEGF)、B-cell lymphoma protein 2(Bcl-2)蛋白水平表达,并用CD105为标记计数微血管密度(microvansicular density,MVD),用TUNEL方法计数凋亡细胞数。结果:(1)COX-2 mRNA在内膜癌中的表达较正常子宫内膜增强(P<0.001)。(2)COX-2蛋白在内膜癌中的表达较正常内膜增强(P<0.001)。(3)COX-2蛋白表达在高、中分化子宫内膜癌组较低分化组升高(P=0.020),与手术分期、病理类型、肌层浸润深度及淋巴结转移无相关性。COX-2 mRNA水平与以上各项因素均无相关性。(4)COX-2的mRNA表达水平与蛋白表达水平无相关性(P=0.125,r=0.222)。(5)COX-2蛋白表达的升高伴有VEGF、MVD和Bcl-2的升高及凋亡细胞减少(P均<0.05)。(6)COX-2的表达增强与ER-α和ER-β蛋白表达无相关性,但与孕激素亚型A和B(PRA、PRB)的表达升高有相关性,P值分别为0.031和0.007。结论:(1)子宫内膜癌中COX-2在mRNA水平及蛋白水平均较正常子宫内膜中明显升高。(2)COX-2的蛋白高表达与肿瘤较好分化相关,而与病理类型、手术分期、肌层浸润深度及淋巴结转移均无相关性。mRNA与以上各因素均无相关性。(3)子宫内膜癌中COX-2的表达增强伴有VEGF蛋白水平升高及微血管密度增加。(4)子宫内膜癌中COX-2的表达增强伴有Bcl-2蛋白水平升高及肿瘤细胞凋亡受到抑制。(5)在内膜癌COX-2阴性与阳性两组间,雌激素受体表达差异无统计学意义,而PRA及PRB在阳性组的表达均较阴性组升高。
Objective：To evaluate the cyclooxygenase-2（COX-2） expression at mRNA and protein le-vels,as well as its clinical significance.and to study the correlation of COX-2 with angiogenesis,apoptosis and estrogen or progestron receptors.Methods：Forty-one samples of normal endometrium tissues and 52 ones of endometrial carcinomas（EC） were collected,together with their corresponding clinical information.RT-PCR was adopted to determine the expression of COX-2 mRNA.Immunohistochemical staining was engaged to evaluate the protein expression of COX-2,VEGF,Bcl-2 and the MVD marked by CD105.And TUNEL was used to count the apoptosis cells.Results：（1） Significantly higher mRNA and protein levels of COX-2 were found in endometrial carcinomas than in normal endometrial tissues（P0.001）.（2） At protein level,COX-2 expression was related to the grade（P=0.020）,but had nothing to do with the stage,pathological type,depth of myometrium invasion and lymph node metastasis.Neither did the level of COX-2 mRNA with all the factors above.（3）The level of COX-2 mRAN had nothing to do with that of protein（P=0.125,r=0.222）.（4） The higher the expression of COX-2 protein was,the higher that of VEGF,MVD or Bcl-2 was,and the lower the count of apoptosis cells was P0.05.（6）The upregulation of COX-2 protein had no correlation with ER-α and ER-β.But it had positive correlation with PRA and PRB,P was 0.031 and 0.007,respectively.Conclusion：（1）The expression of COX-2 is significantly higher in EC than in normal endometrium tissue,not only at the mRNA level,but also at protein level.（2）At protein level,COX-2 expression is related to the grade,but has nothing to do with the stage,pathological type,depth of myometrium invasion and lymph node metastasis.Neither does the level of COX-2 mRNA with all the factors above.（3）The upregulation of COX-2 protein in EC correlates with the increase of VEGF protein and MVD.（4）The upregulation of COX-2 protein in EC correlates with the increase of Bcl-2 protein and the decrease of apoptosis cells.（5）The upregulation of COX-2 protein in EC has no significant correlation with ER expression,but has something to do with the increase of PRA and PRB.
出处
《北京大学学报（医学版）》
CAS
CSCD
北大核心
2009年第6期657-663,共7页
Journal of Peking University:Health Sciences
基金
国家自然科学基金(30172225)
教育部科学技术研究重大项目(0104)资助~~
作者简介
Corresponding auther＇ s e-mail, xindichen@ sina, com
