摘要
目的:探讨血管紧张素Ⅱ(AngⅡ)诱导人单核/巨噬细胞(THP-1细胞)基质金属蛋白酶-9(MMP-9)表达的机制。方法:用0.1μmol/L佛波酯(PMA)作用48h,诱导THP-1单核细胞分化为巨噬细胞后,随机分为4组:(1)PMA组,即对照组;(2)PMA+AngⅡ组(10-7mol/L,1h);(3)PMA+AngⅡ+2-硫代氨基甲酸吡咯烷组[PDTC(10μmol/L,30min)];(4)PDTC组。用Western blotting蛋白印记技术分别检测总蛋白MMP-9及磷酸化核转录因子-κBp65(NF-κBp65)的变化,用RT-PCR检测MMP-9 mRNA表达的变化。结果:与对照组相比,AngⅡ诱导THP-1巨噬细胞磷酸化NF-κBp65增加(1.02±0.10,P<0.05),MMP-9蛋白量也增加(1.06±0.11,P<0.05),MMP-9 mRNA表达上调(1.22±0.08,P<0.05),而使用NF-κB的抑制剂PDTC后磷酸化NF-κBp65(0.99±0.12,P<0.01)减少,MMP-9表达明显受到抑制(1.04±0.14,P<0.01),MMP-9 mR-NA表达下调(0.90±0.06,P<0.01)。结论:NF-κB信号转导途径是AngⅡ诱导THP-1巨噬细胞表达MMP-9的重要途径之一。
AIM： The present study was undertaken to investigate the effect of angiotensin Ⅱ （Ang Ⅱ ） on expression of MMP - 9 in THP - 1 macrophages. METHODS ： Macrophages converted from THP - 1 monocytes by incubating with PMA （0. 1 μmol/L） for 48 h were divided into PMA group; PMA ＋ Ang Ⅱ group （ 10-7mol/L, 1h） ; PMA ＋ Ang Ⅱ ＋ PDTC group （10 μmol/L, 30 min） and PDTC group. Western blotting was used to detect the MMP- 9 and phosphorylation of NF - kB p65, and the expression of MMP - 9 mRNA in THP - 1 macrophages was measured by RT - PCR. RESULTS： Compared to control group, the expression of MMP - 9 （ 1.06 ± 0. 11, P 〈 0. 05 ） and phosphorylation of NF - kB p65 （ 1.02 ± 0. 10, P 〈 0.05 ） in THP - 1 macrophages were expressed when treated with Ang Ⅱ （ 10 -7 mol/L） ; and the expression of MMP - 9 mRNA were upregulated （ 1.22 ±0.08, P 〈 0. 05 ）. However, NF - kB inhibitor PDTC reduced the NF - kB p65 （0. 99 ±0. 12, P 〈0. 01） and MMP -9 （ 1.04 ±0. 14, P 〈0. 01 ） expressions and decreased the expression of MMP -9 mRNA （0.90 ±0.06,P 〈0. 01 ）. CONCLUSION： NF - KB signaling pathway contributes to the expression of MMP - 9 in THP - 1 macroohage induced by Ang Ⅱ.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2009年第12期2334-2337,共4页
Chinese Journal of Pathophysiology
作者简介
通讯作者 E—mail：zhimingyang800@sina．com
