摘要
利用人工水质染毒的方法,采用组织切片、透射电镜和单细胞凝胶电泳(彗星实验)技术,观察小鼠卵母细胞的超微结构变化及DNA损伤状况,从形态学角度研究不同剂量铬对小鼠雌性生殖细胞凋亡及DNA损伤的影响,目的在于探讨三价铬的繁殖毒性。实验设置对照组及3个处理实验组,分别饮用添加0.24、0.48和0.72 mmol·L^(-1)三氯化铬的纯净水,对照组小鼠饮用纯净水,30 d后取材观察。结果表明:除实验一组外,各实验组中小鼠卵母细胞处于凋亡时期的数量显著高于对照组,凋亡时期的卵母细胞超微结构呈现出线粒体空泡、核膜内陷、染色质周缘化及核固缩等现象。高剂量处理(0.72 mmol·L^(-1))组彗星比率为21.35%、尾部DNA含量为17.53%、Olive尾矩(OTM)为3.92,均显著高于对照组,而头部DNA含量(82.47%)显著低于对照组(94.15%)(P<0.05),说明添加高剂量铬容易引起小鼠卵巢发育过程中卵母细胞DNA的损伤。
The purpose of present study was to examine the Reproductive toxicity of trivalent chromiumto. By artificial water contamination, tissue slice, transmission electron microscope （TEM） and single cell gel electrophoresis （SCGE） were made to investigate the effect of chromium on oocyte apoptosis in mice ovary., as well as the damage at DNA. In this experiment, mice were divided randomly into four groups, CrCl3 was given to three groups perorally at the dose of 0.24, 0.48 and 0.72 mmol·L^-1. Purified water was given to the left group as the control. After 30-day feeding, ooeytes were taken for observation. The results showed that the amount of oocyte apoptosis in treatment groups mice was remarkably higher than the controlled group. The ultrastructure of oocytes in different periods of apoptosis showed the phenomena such as mitochondrion vacuole, karyotheca invagination, chromatin margination and karyopyknosis. Compared with the controlled group, treatment group of 0.72 mol·L^-1 dose was the highest, remarkably higher in comet ratio （21.35%）, comet tail DNA content （17.53%） and the tail moment （OTM） values （3.92）. And comet head DNA content （82.47%） was remarkably lower than the controlled gro up （94.15%）（P〈0.05）. All above indicated that chromium can cause oocyte apoptosis in mice easily.
出处
《上海交通大学学报（农业科学版）》
2009年第6期561-565,共5页
Journal of Shanghai Jiaotong University(Agricultural Science)
基金
上海市科委自然基金项目(ZR0714056)
上海交通大学PRP项目(T15014034)
关键词
铬
小鼠卵母细胞
DNA损伤
凋亡
trivalent chromium
mouse oocyte
DNA damage
apoptosis
作者简介
王亮亮（1984-），男，河北邯郸人，硕士研究生，研究方向：动物繁殖学
通讯作者：李新红 E-mail：Lixinhong7172@sjtu．edu．cn
