摘要
目的:分析非小细胞性肺癌(NSCLC)中Runx3基因启动子区甲基化状态。方法:运用甲基化特异性PCR技术检测62例NSCLC组织和癌旁正常肺组织中Runx3基因启动子甲基化,并分析该基因启动子甲基化Runx3基因mRNA和蛋白表达的影响及其与临床特征之间的关系。结果:NSCLC组织中Runx3基因异常甲基化率(48.4%)显著高于癌旁正常肺组织中Runx3基因的异常甲基化率(17.7%,P=0.000);发生完全或者不完全甲基化的NSCLC组织或者正常肺组织中Runx3基因mRNA和蛋白表达显著降低。Runx3基因启动子区高甲基化和肿瘤分化程度及临床分期有相关性(P=0.041和0.009),而与NSCLC患者性别、年龄、有无吸烟史及肿瘤类型等临床特征无关(P=0.400,0.301,0.290和0.965)。结论:Runx3基因启动子区异常甲基化是导致Runx3基因在NSCLC中表达下调的重要因素,有望成为NSCLC早期辅助诊断的分子标志物之一。
Objective：To analyze the aberrant promoter hypermethylation of Runx3 gene in human non-small cell lung carcinoma（NSCLC）.Methods： Methylation-specific PCR was performed to detect the promoter hypermethylation of Runx3 gene in 62 NSCLC tissue samples and 62 corresponding normal lung tissue samples.The effect of the aberrant methylation of Runx3 gene on the expression of Runx3 mRNA and protein was evaluated.The correlation between the aberrant methylation of Runx3 gene and the clinicopathological factors of NSCLC patients was also analyzed. Results： The promoter hypermethylation ratio of Runx3 gene in NSCLC tissues （48.4%） was significantly higher than that of the corresponding normal lung tissues （17.7%, P=0.000）. The levels of p16 mRNA and protein expression in NSCLC tissues or normal lung tissues with complete or incomplete methylation were significantly lower than those with unmethylation. The aberrant methylation of Runx3 promoter was significantly correlated with tumor differentiation （P=0.041） and clinical stage （P=0.009）, but not correlated with sex, age, smoking condition and tumor types of NSCLC patients （P=0.400, 0.301, 0.290 and 0.96, respectively）. Conclusion： The aberrant methylation of Runx3 gene was associated with Runx3 downregulation, and detection of the aberrant methylation of Runx3 gene promoter in NSCLC would offer an effective method for the earlier auxiliary diagnosis of NSCLC.
出处
《现代生物医学进展》
CAS
2009年第19期3692-3695,共4页
Progress in Modern Biomedicine
作者简介
侯道荣（1979-），男，硕士，主要从事肿瘤发生的分子机制研究和动物模型建立电话：025—86862061；Email：hdr_nanjing@163．com
