摘要
目的:探讨一种新的睾丸特异性蛋白FSCB(Fibrous sheath CABYR binding protein)在精子内的精细动态表达和迁移过程,并研究该蛋白在正常和畸形精子中的形态定位特征。方法:应用过碘酸-希夫氏染色获得鼠睾丸曲精小管上皮中精子发生的时相性背景特征,然后应用间接免疫荧光方法对FSCB蛋白在睾丸和精子内的动态表达和迁移过程进行观察。结果:FSCB最初表达于长精细胞的第11步(时相Ⅺ),呈胞浆内弥漫性染色;随之从第11步(时相Ⅺ)至第15步(时相Ⅵ)表达逐渐加强;在精子形成的第15步(时相Ⅳ至时相Ⅵ)FSCB开始向精子鞭毛迁移,最终于第16步(时相Ⅷ)FSCB蛋白整体局限定位于睾丸精子鞭毛上,胞浆内荧光染色消失。进一步研究显示该蛋白在正常精子中定位于鞭毛主段、精子头部、鞭毛颈段、中段和末段均无表达。在畸形精子中,该蛋白表达存在3种异常改变:①鞭毛内无阳性表达;②表达节段前移并缩短,提示这类精子缺乏中段;③呈现间断分布的串珠状改变。结论:FSCB是纤维鞘的重要组成蛋白,在精子纤维鞘形成的后期装配于其表面,可能是纤维鞘生物合成的最后装配步骤。该蛋白在畸形精子中存在明显表达异常,其表达异常与精子运动功能之间的关系有待深入研究。
Objective：To explore the dynamic expression and migration of the novel testis- and sperm-specific protein FSCB in mouse spermatogenesis and observe the morphological localization of FSCB on normal and abnormal mouse sperm. Methods： For exactly staging of the mouse tubule sections, Periodic acid-Schiff staining was performed at first, and then the dynamic expression of FSCB on mouse testis and sperm by indirect Immunofluorescence was observed. Results：FSCB is a post meiotic protein initially expressed at step 11（stage Ⅺ）of mouse spermatogenesis in the elongating spermatids, and it’s expresson gradully become stronger and stronger from step 11（stage Ⅺ） through step 15 （stage Ⅵ）. It subsequently begin migrate into the flagellar at step 15, and at last totally localize to the sperm flagellar at step 16（stage Ⅷ）accompanied with its disappearing from the cytoplasma of sperm. The exact localization of FSCB on normal sperm is in the flagellar principal piece. Its expression and localization on abnormal sperm was variablely changed：a.No expression on the whole flagellar;b. The expression segment on the flagellar was shift to near the head and become short, suggest this sperm defect middle piece;c. Its distribution on the flagellar showed beads like shape. Conclusion： FSCB is an important constituent protein of the fibrous sheath, is assembled at the surface of fibrous sheath core structure and constitutes the final assembly step of fibrous sheath at the later stages of spermiogenesis. The expression of FSCB in abnormal sperm was significantly changed. The relationship between the abnormal expression of FSCB and the sperm motility need to be further studied.
出处
《重庆医科大学学报》
CAS
CSCD
北大核心
2009年第9期1171-1175,共5页
Journal of Chongqing Medical University
基金
第三军医大学留学回国人员启动基金资助
关键词
精子
纤维鞘
精子发生
睾丸特异性蛋白
Sperm Fibrous sheath Spermatogenesis Testis-specific protein
作者简介
李彦锋（1967～），男，副教授，博士，研究方向：男性生殖生理和避孕。
通讯作者：何畏，女，副教授，E—mail：anyhewei@yahoo．com．cn。
