摘要
【目的】比较滩羊、蒙古羊和小尾寒羊BMPR-IB基因的多态性,分析不同基因型对产羔数的影响,为绵羊繁殖力的标记辅助选择和育种提供理论依据。【方法】利用PCR-RFLP方法检测和分析BMPR-IB基因的多态性,通过最小二乘分析方法研究不同基因型对产羔数的影响。【结果】(1)滩羊和小尾寒羊均发现了++、B+和BB3种基因型,其基因型频率分别为0.660,0.240,0.100和0.118,0.353,0.529,+和B的基因频率分别为0.780,0.220和0.294,0.706,蒙古羊仅表现++1种基因型;滩羊、小尾寒羊BMPR-IB基因位点的多态信息含量(PIC)分别为0.439和0.501,表现为中度多态(0.25<PIC<0.5)和高度多态(PIC>0.5);(2)小尾寒羊BB基因型个体平均产羔数较B+基因型多0.541只,差异达显著水平(P<0.05),较++基因型多1.208只,差异达极显著水平(P<0.01);滩羊BB基因型个体平均产羔数较B+和++基因型分别多0.076和0.159只,差异均不显著(P>0.05)。【结论】(1)小尾寒羊和滩羊BMPR-IB基因编码序列第746位相邻2个碱基处发生了与布鲁拉美利奴绵羊(Booroola Merino)相同的突变(A746G);(2)BMPR-IB基因位点的突变基因B可能是控制小尾寒羊和滩羊多胎性能的主效基因,或者是与之紧密连锁的遗传标记,可以用于提高繁殖力的标记辅助选择和育种工作。
[Objective] The study compared the gene polymorphism of BMPR-IB gene in the Tan sheep, Mongolian Sheep and Small Tailed Han Sheep,and analyzed the effect on the litter size of the different genotype,which would provide a theoretical basis for marker-assisted selection and breeding for prolificacy in sheep. [Method] The gene polymorphism of BMPR-IB gene was analyzed by PCR-RFLP and the effect on litter size of different genotypes was studied by least squares mean. [Result] （1）There were three genotypes ＋＋,B＋ and BB in the Tan Sheep and Small Tailed Han Sheep, the genotype frequencies were 0. 660,0. 240,0. 100 and 0. 118,0. 353,0. 529 respectively,0. 780,0. 220 and 0. 294,0. 706 were gene frequencies of ＋ and B,and only ＋＋ genotype existed in Mongolian Sheep. The polymorphism information contents （PIC） were 0. 439 and 0. 501 in the Tan Sheep and Small Tailed Han Sheep,which means moderate diversity （0.25〈PIC〈0. 5）and high diversity （PIC〉0. 5） respectively. （2）The average litter size of Small Tailed Han Sheep with genotype BB had 0. 541 （P〈0.05） and 1. 208 （P〈0.01） lambs more than B＋ and ＋＋ genotypes. Tan Sheep were more than 0. 076 and 0. 159（P〉0.05） lambs. [Conclusion] （1） There is the same mutation in BMPR-IB gene＇ s consecutive two basic （A746G） of Small Tailed Han Sheep and Tan Sheep as that of the Booroola Merino; （2） The B gene of BMPR-IB gene mutation locus may be a major gene which affects the prolificacy trait of Tan Sheep and Small Tailed Han Sheep or it is regarded as a genetic makers closely linked to target gene and could be as a marker assisting selection and breeding of improve fecundity.
出处
《西北农林科技大学学报（自然科学版）》
CSCD
北大核心
2009年第11期31-36,共6页
Journal of Northwest A&F University(Natural Science Edition)
基金
宁夏滩羊选育及养殖配套技术与示范推广项目
作者简介
[作者简介]田秀娥（1964-），女，陕西泾阳人，副教授，硕士生导师，主要从事动物遗传育种与繁殖研究。E-mail：txe82@yahoo．com．cn
[通信作者]王永军（1964-），男，陕西礼泉人，副教授，硕士生导师，主要从事动物遗传育种与繁殖研究。E-mail：dkxywyj2008@yahoo．com．cn
