摘要
目的:探讨褪黑素(Melatonin,MLT)对慢性阻塞性肺疾病(Chronic obstructive pulmonary disease,COPD)大鼠模型气道氧化应激的影响。方法:36只雄性Wistar大鼠随机分成对照组、COPD组、MLT组,采用熏烟加气管注入脂多糖(Lipopolysaccharide LPS)复制COPD大鼠模型,MLT组在造模前30min腹腔注入MLT。28d后气管切开测定肺功能,检测肺泡灌洗液(BALF)中白细胞计数及分类,BALF中一氧化氮(NO)、丙二醛(MDA)、总超氧化物歧化酶(T-SOD)、谷光甘肽过氧化物酶(GSH-PX),以及光镜下观察病理形态。结果:(1)MLT组BALF中白细胞总数、中性粒细胞比例较COPD组明显减低(分别为P<0.01,P<0.05),单核-巨噬细胞绝对计数下降,与对照组相比差异均无统计学意义。(2)MLT组大鼠BALF中NO、MDA较COPD组明显减低(P<0.05),T-SOD、GSH-PX较COPD组明显增高(P<0.05)。(3)MLT组0.3s用力呼气容积(FEV0.3)、0.3s用力呼气容积/用力肺活量(FEV0.3/FVC)、峰流速(PEF)较COPD组均有增加,但两者之间无显著性差异(P>0.05),同时仍较对照组减低(P<0.05)。(4)COPD组支气管、肺实质病理改变具有特征性,MLT组病理损害减轻,但仍异常于对照组。结论:通过减少氧化应激,增加抗氧化酶类的活性,MLT早期干预能够部分阻止COPD的进程。
Objective：To investigate the effect of melatonin（MLT） on airway oxidative stress in rats with chronic obstructive pulmonary disease（COPD）.Methods：36 male wistar rats were randomly divided into 3 groups as follows：control group,COPD group and MLT group.The COPD model was established by intratracheal lipopolysaccharide（LPS） and exposure to cigarette smoking for 28 days,the MLT group was given intraperitoneal injection of 10 mg/kg MLT 30 minutes before each cigarette challenge.After incision of trachea,the lung function was measured.Then the rats were lavaged,the total and different white blood cell counts of bronchial alveolar lavage fluid（BALF） were determined;nitric oxide（NO）,malondialdehyde（MDA）,superoxide dismutase（T-SOD）,glutathione superoxide（GSH-PX） were carried out respectively.The pathological changes were observed under light microscope.Result ：（1）Significant decrease in total white blood cells and neutrophils in BALF was found in MLT group than these in COPD group（P〈0.01,P〈0.05,respectively）,and the ratio of monocyt-macrophage was lower in MLT group.（2） NO and MDA in BALF of MLT group significantly decreased（P〈0.05）,while T-SOD and GSH-PX in BALF of MLT group significantly increased（P〈0.05）,as compared with those of COPD group.（3） Forced expiratory volume in 0.3 second（FEV0.3）,forced expiratory volume in 0.3 second /forced vital capacity（FEV0.3/FVC） and peak expiratory flow（PEF） in MLT group were higher than those in COPD group,but there were no obvious differences between these groups.（4） The model rats shared specific pathological features in bronchial walls and lung tissues,and the pathological damages in MLT group were lessened but still abnormal.Conclusion：Early intervention with MLT can partly inhibit proceeding of COPD by decreasing airway oxidative stress and increasing activity of antioxidant.
出处
《重庆医科大学学报》
CAS
CSCD
北大核心
2009年第8期1046-1050,共5页
Journal of Chongqing Medical University
关键词
肺疾病
慢性阻塞性
氧化应激
褪黑素
Puhnonary disease
Chronic obstructive
Oxidative stress
Melatonin
作者简介
夏峻巍（1972-），男，主治医师，硕士，研究方向：慢性阻塞性肺疾病。
通讯作者：徐玲，女，主任医师，E—mail：vincentxia007@yahoo．com．cn。
