摘要
目的探讨骺板不同部位损伤对骺板生长发育的影响。方法选用新西兰白兔96只,估算骺板损伤面积百分比,随机分组制备股骨远端骺板中央及外侧边缘区损伤模型,术后不同时间从大体和组织学观察骺板发育情况。结果直径2.0mm、3.0mm和3.5mm组骺板损伤面积百分比分别为(3.33±0.42)%、(6.77±0.23)%和(9.05±0.30)%。术后骺板中央区损伤3.0mm组和3.5mm组出现生长阻滞,而外侧边缘区损伤3.5mm组出现生长阻滞;外侧边缘区损伤3.0mm组和3.5mm组出现髁部发育阻滞及外翻成角畸形。组织学观察发现骺板损伤区骨桥形成,软骨细胞呈"岛状"分布,排列不规则,周围被骨桥包绕。结论骺板发育阻滞与损伤面积百分比有关,中央区损伤主要引起肢体短缩畸形,而外侧边缘区损伤主要引起肢体成角畸形及骺板横向发育阻滞;骺板损伤后其损害是永久性的;骺板损伤后软骨细胞修复能力有限。
Objective To explore the effect of penetrating injuries of different regions on the development of epiphyseal plate of immature rabbits. Methods 96 New Zealand white rabbits were used in the experiment, the percentage of areas of epiphyseal plates of both femora were estimated before operations. All the 96 rabbits were allocated into two groups randomly, Kirschner wires with different diameters were used to penetrate the central or lateral marginal regions of right distal femoral epiphyseal plate of rabbits, and the left side as control. All animals were observed at certain time points to detect the changes on the whole sample and histopathology of the development of epiphyseal plate. Results The percentage of injuried epiphyseal plates in three groups were 3.33±0.92%, 6.77±0.23 %,9.05±0.30 %. In the 12 weeks observation, the growth retardation were appeared in the groups （3.0mm and 3.5mm） of central epiphyseal plate injuries and in 3.5mm group of lateral marginal epiphyseal plate injuries. The transverse diameter of condyles of femur was diminished in the groups （3.0mm and 3.5mm） of lateral marginal epiphyseal plate injuries and it showed growth retardation and valgus angulation deformities in condyles of femur. Bone bridges were found in all injury regions of epiphyseal plates through the analysis of pathohistology and immunohistochemistry, the chondrocytes of epiphyseal plate adjacent to injury regions were scattered as ＂island of cartilage＂ and the chondrocytes aligned irregularly surrounded by bony tissue bridges. Conclusion The epiphyseal plate retardation secondary to a penetrating injury from Kirschner wire is related to the percentage of the injured area. The injuries in central regions of epiphyseal plates mainly induced limb shortening and injuries in lateral marginal regions mainly caused deformities of valgus angulation and transversal developmental arrest of epi- physeal plate. The fibro-vasa bridges or bone bridges were seen in all the injury regions of epiphyseal plates. The chond- rocytes of epiphyseal plate had the repare ability after injury, but the ability was limited.
出处
《生物骨科材料与临床研究》
CAS
2009年第5期7-12,共6页
Orthopaedic Biomechanics Materials and Clinical Study
关键词
骺板
骨骺
损伤
动物实验
Epiphyseal plate
Epiphysis
Trauma
Animal experiment
作者简介
陈梓锋（1976-）男，硕士，主治医师，研究方向：骨创伤与修复重建外科。
[通信作者]陈爱民（1963-）男，教授，博士生导师，研究方向：骨创伤与修复重建外科，E-mail：chenaimin6403@yahoo．com．cn
