摘要
目的建立高效液相色谱法检测酶促反应液中L-半胱氨酸含量。方法采用异硫氰酸苯酯柱前衍生高效液相色谱法测定。色谱柱：Waters Symmetry C18（5μm，250mm×4．6mm）；流动相A：醋酸钠缓冲溶液-乙腈（40：3），流动相B：乙腈-水（4：1）；梯度洗脱，流速1．0mL／min；柱温38℃；检测波长254nm。结果线性范围为0．01～0．1mg／mL，r=0．9995（n=6）；平均回收率为100．03％，RSD为0．21％（n=6）。结论此法简便易行。
Purpose To establish a HPLC method for the content determination of L-cysteine in enzymatic reaction mixture. Methods L-cysteine was derivatized with phenylisothiocyanate, and the content of L-cysteine in enzymatic reaction mixture was determined by HPLC. The column was Waters Symmetry C18 （5μm,250 mm × 4.6 mm）.The mobile phase A was sodium acetate buffer-acetonitrile（40： 3）, and the mobile phase B was acetonitrile-water（4：1 ）. The flow rate was 1.0 mL/min. Detection wavelength was 254 nm and the column temperature was 38 ℃. Results The linear range was 0.01-0.1 mg/mL, r = 0.999 5（ n = 6）. The average recovery was 100.03 % ,and RSD was 0.21% （n = 6）. Conclusion The method is simple, rapid ,accurate and reliable.
出处
《中国生化药物杂志》
CAS
CSCD
北大核心
2009年第5期318-320,共3页
Chinese Journal of Biochemical Pharmaceutics
作者简介
张苏（1979-），女，山东邹平人，工程师，主要从事生物制药分析研究工作
王凤山，通信作者，Tel：0531-88080288，E-mail：fswang@sdu．edu．cn。
