摘要
Ｒ２８４．１；Ｒ３３１．３８；Ｒ９７２．２；Ｒ９７２．４摘要目的观察Ｒｂ１对豚鼠心肌细胞膜Ｌ－型电压依赖性钙通道的影响。方法采用标准全细胞膜片钳技术（ｗｈｏｌｅ－ｃｅｌｐａｔｃｈｃｌａｍｐｒｅｃｏｒｄｉｎｇｔｅｃｈｎｉｑｕｅ）。结果在保持电位－４０ｍＶ，细胞去激化至＋４０ｍＶ，刺激频率０．５Ｈｚ，时程１５０ｍｓ条件下，Ｒｂ１１０μｍｏｌＬ－１和３０μｍｏｌＬ－１分别使ＢａｙＫ８６４４和ｎｉｆｅｄｉｐｉｎｅ敏感的钙内向电流减少１６．２％±３．７％（Ｐ＜０．０５，ｎ＝５）和３８．３％±１０．４％（Ｐ＜０．０１，ｎ＝５），且在３～１０００μｍｏｌＬ－１范围内，其抑制作用呈浓度依赖关系。结论实验证明Ｒｂ１为一钙通道阻滞剂。
AIM To evaluate the effects of Rb1 on LCa2+ channel of guinea pig ventricular myocytes. METHODS The wholecell patch clamp recording technique when the holding potential was -40 mV, the cell was depolarized to +40 mV for 150 ms at frequency of 05 Hz. RESULTS Rb1 10 μmol·L-1 and 30 μmol·L-1 reduced Bay K 8644 and nifedipinesensitive Ltype Ca2+ inward current 162%±37%(P<005, n=5) and 383%±104%(P<001, n=5) respectively. The effects of Rb1 on Ca2+ current reached the maximun at 10 min after Rb1 was added. CONCLUSION It is suggested that Rb1 should be a Ca2+ channel antagonist.
出处
《中国药理学通报》
CAS
CSCD
北大核心
1998年第1期33-35,共3页
Chinese Pharmacological Bulletin
基金
云南省卫生厅中医中药管理处研究基金
关键词
三七皂甙
RB1
心肌细胞膜
钙通道
panaxadiol
ginseng
saponins
Rb 1
patchclamp
calcium channel
