摘要
目的:研究香加皮杠柳苷(CPP)对荷瘤小鼠的免疫调节作用及其作用机制。方法:建立BALB/c小鼠H22皮下移植瘤,观察低、中、高剂量CPP(0.25、0.50、1.00mg/kg)对荷瘤小鼠免疫器官的影响,应用流式细胞术检测各组小鼠脾T淋巴细胞亚群的分布,应用MTT法检测ConA诱导的小鼠脾淋巴细胞增殖活性,用ELISA试剂盒测定各组荷瘤小鼠血清中细胞因子TNF-α、IL-2和IL-12的含量。结果:对照组荷瘤小鼠的胸腺指数及脾脏指数均明显低于未荷瘤正常小鼠(P<0.05),而CPP组荷瘤小鼠的胸腺指数及脾脏指数较对照组明显增高,甚至高于正常对照组(P<0.05)。CPP对荷瘤小鼠体内CD8+T细胞数量无影响,但可明显上调CD3+、CD4+T细胞数及CD4+/CD8+比值,其中CD3+、CD4+细胞百分率与正常小鼠无差别(P>0.05),CD4+/CD8+比值高于正常小鼠(P<0.05)。CPP可明显增强ConA诱导的荷瘤小鼠脾淋巴细胞的增殖能力,SI甚至超过正常对照组(P<0.05)。不同剂量CPP组小鼠血清中TNF-α、IL-2和IL-12水平均较对照组明显增高(P<0.05),并随剂量增加呈升高趋势,至接近或超过正常小鼠水平。结论:CPP可保护荷瘤小鼠的免疫器官不受损害,并可明显提高CD4+T细胞百分率和CD4+/CD8+比值,增强荷瘤小鼠T细胞增殖能力,促进细胞因子TNF-α、IL-2和IL-12的产生,表明CPP具有显著的免疫增强作用。
AIM： To investigate the immune mechanisms for Periplocin from Cortex Periplocae （CPP） in tumor-bearing mice. METHODS： H22 tumor-bearing model BALB/c mice were applied to evaluated in vivo immunoregu- latory effect of CPP. The influence of different dose CPP （0.25, 0.50 and 1.00 mg/kg） on immune organs in tumorbearing mice were observed. T cell subsets of mice spleen were detected by flow cytometry. MTT assay was used to determine the influence of CPP on lymphocyte proliferation of mice spleen stimulated by ConA. The levels of TNF-α, IL-2 and IL-12 in serum from mice were detected by means of ELISA. RESULTS： Thymus index and spleen index of H22 tumor-bearing model control mice became less than that of normal mice （ P 〈 0.05）. Compared to both model and normal control groups, thymus index and spleen index of H22 tumor-bearing mice treated with CPP increased obviously（P〈0.05）. CPP had no influence on the number of CD8 ^＋ T cells, but up-regulated markedly the number of CD3 ^＋ , CD4^＋ T cells and the ratio of CD4 ^＋/CD8^＋ in tumorbearing mice. In CPP-treated mice, the percentage of CD3 ^＋, CD4 ^＋T cells were not different from normal mice （P〈0.05）, the ratio of CD4 ^＋/CD8 ^＋ was higher than that of normal mice （P〈0.05）. CPP enhanced obviously lymphocyte proliferation of mice spleen induced by ConA, the SI scores were even higher than that of nornal mice. The levels of TNF-α, IL-2 and IL-12 in serum from CPP-treated mice, increased significantly compared to model control group （P 〈 0.0.5） in a dose-dependent manner, were similar to or higher than that of normal mice. CONCLUSION： CPP protected immune organs of tumor-bearing mice, increased obviously the percentage of CD4^＋ and CD4^＋/ CD8^＋ among the T cell line, and enhanced lymphocyte proliferation of mice spleen significantly, stimulated the production of TNF-αa, IL-2 and IL-12. The results suggested that CPP possessed potent immunoregulatory effect.
出处
《细胞与分子免疫学杂志》
CAS
CSCD
北大核心
2009年第10期887-890,共4页
Chinese Journal of Cellular and Molecular Immunology
基金
国家自然科学基金资助项目(30772752)
河北省自然科学基金资助项目(C2004000610)
关键词
香加皮杠柳苷(CPP)
荷瘤小鼠
免疫调节
Periplocin from Cortex Periplocae （CPP）
tumor-bearing mice
immunoregulation
作者简介
张静（1972-），女，河北正定人，副主任医师，博士
Corresponding author。E-mail：baoenshan@yahoo．com．cn
