期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

腺病毒介导的bFGF基因对大鼠大脑中动脉闭塞模型的作用 被引量：1

Effects of adenovirus mediated bFGF gene on the middle cerebral artery occlusion in rats
原文传递
导出
摘要 采用改良的zea Longa法制备大鼠脑缺血模型,研究腺病毒介导的bFGF的基因治疗对大鼠大脑中动脉闭塞(MCAO)模型的治疗作用及脑血管发生的影响.将30只SD大鼠随机分为假手术组、空载体组(Ad-null组)和治疗组(Ad-bFGF组),于造模成功后24h尾静脉给药.检测实验各组脑组织含水率的变化,通过核磁共振的和免疫组化分别检测实验各组大脑梗死体积和血管发生的变化.结果发现治疗组大鼠脑组织含水率和脑梗死面积明显小于空载体组,脑血管密度显著高于空载组.说明腺病毒介导的bFGF基因可显著减小脑组织含水率和脑梗死面积,促进脑部血管发生. Thirty rats were randomly divided into three groups, i. e. , sham group, control group （Adnull group） and drug treatment group （Ad-bFGF group）. To study the effect of Adenovirus mediated bFGF gene on the middle cerebral artery occlusive rats model and treated in acute ischemic cerebrovascular disease. The model of middle cerebral artery occlusion in rats was established successfully by Zea Longa method. Twenty-four hours later, Ad-bFGF was given to the rats in Ad-bFGF group through intravenous injection. The Ad-null was given to the rats of the control group. The water content, the rats＇ ischemic volume and the change of microvessel density in the brain of middle cerebral artery occlusion rats were observed. Compared with the control group, the water ratio and the rats＇ ischemic volume of the drug treatment group decreased significantly and the microvessel density increased remarkably. The Adenovirus mediated bFGF gene has obvious effect on the decreasing the water ratio and the rats＇ ischemic volume, and promoting angiogenesis.
作者 刘康 杨莉 李洁 白瑞珍 刘劝 胡火珍 宋桂芹
机构地区 四川大学生命科学学院 四川大学华西医院生物治疗国家重点实验室 川北医学院医学生物学教研室
出处 《四川大学学报（自然科学版）》 CAS CSCD 北大核心 2009年第5期1478-1482,共5页 Journal of Sichuan University(Natural Science Edition)
基金 国家重点基础研究发展计划(973)资助项目(2005CB522506)
关键词 大脑中动脉闭塞 BFGF 重组腺病毒载体 基因治疗 MCAO, bFGF, recombinant adenovirus vector, gene therapy
分类号 R-33 [医药卫生]
作者简介 刘康（1983-），男，硕士，研究方向：新生血管及肿瘤的生物治疗，E-mail：liukangscu@163．com 通讯作者：胡火珍．E-mail：huhuozhen@163．com
  • 相关文献

参考文献12

  • 1Grothe C, Unsicker K. Basic fibroblast growth factor in the hypoglossal system: specific retrograde transport, trophic, and lesion related responses [J]. Neurosci Res, 1992, 32 (3): 317.
  • 2Rosenblatt S, Irilura K, Caday C G, et al. Basic fibroblast growth factor (bFGF) dilates rat pial arterioles[J]. J Cereb Blood Flow Metab, 1994, 14(1): 70.
  • 3Heldin C H, Eriksson U, Ostman A. New members of the platelet-derived growth factor family of mitogens. Arch Biochem Biophys, 2002, 398: 284.
  • 4Zea-longa E, Weinstein P R, Carlson S, et al. Reversible middle cerebral artery occlusion without craniectomy in rats [J]. Stroke, 1989, 20 (1): 84.
  • 5Zausinger S, Hungerhuber E, Baethmann A, et al. Neurological impairment in rats after transient middle cerebral artery occlusion: a comparative study under various treatment paradigms [J]. Brain Res, 2000, 863(1-2): 94.
  • 6Bohlen P, Baird A, Esch F, etal. Isolation and partial molecular characterization of pituitary fibroblast growth factor [J]. Proc Natl Acad Sci USA, 1998, 81(17) : 5364.
  • 7Fujiwara K, Date L, Shingo T, et al. Reduction of iNTParct volume and apoptosis by grafting of encapsulated basic fibroblast growth factor-secreting cells in a model of middle cerebral artery occlusion in rats [J]. J Neurosurg, 2003, 99(5): 1053.
  • 8Brewer G J, Espinosa J A, Struble R G, et al. Effect of Neuregen nutrient medium on survival of cortical neurons after aspiration lesion in rats [J]. J Neurosurg, 2003, 98(3) : 1291.
  • 9Li Q, Stephenson D. Postischemic administration of basic fibroblast growth factor improves sensorimoter function and reduces infarct size following permanent focal cerebral ischemia in the rat[J]. Exp Neurol, 2002, 177 (2): 531.
  • 10Sugimori H, Speller H, Finklestein S P. Intravenous basic fibroblast growth factor produces a persistent reduction in infarct volume following permanent focal ischemia in rats[J]. Neurosci Lett, 2001, 300 (1): 13.

同被引文献26

  • 1Chan J,O'Donoghue K,de la Fuente J. Human fetal mesenchymal stem cells as vehicles for gene delivery[J].Stem Cells,2005,(01):93-102.
  • 2Sun L,Cui M,Wang Z. Mesenchymal stem cells modified with angiopoietin-1 improve remodeling in a rat model of acute myocardial infarction[J].Biochemical and Biophysical Research Communications,2007,(03):779-784.
  • 3Liang XJ,Chen XJ,Yang DH. Human umbilical cord mesenchymal stem cells by hTERT Gene Transfection can differentiate into hepatocyte-like cells in vitro[J].Cell Biology International,2011.20110350.
  • 4Tsai CC,Chen CL,Liu HC. Overexpression of hTERT increases stem-like properties and decreases spontaneous differentiation in human mesenchymal stem cell lines[J].Journal of Biomedical Science,2010.64.doi:10.1186/1423-0127-17-64.
  • 5McMahon JM,Conroy S,Lyons M. Gene transfer into rat mesenchymal stem cells:a comparative study of viral and nonviral vectors[J].Stem Cells and Development,2006,(01):87-96.
  • 6Su K,Wang D,Ye J. Site-specific integration of retroviral DNA in human cells using fusion proteins consisting of human immunodeficiency virus type 1 integrase and the designed polydactyl zinc-finger protein E2C[J].Methods:A Companion to Methods in Enzymology,2009,(04):269-276.
  • 7Madeira C,Mendes RD,Ribeiro SC. Nonviral gene delivery to mesenchymal stem cells using cationic liposomes for gene and cell therapy[J].J Biomed Biotechno,2010.735349.
  • 8Kulamarva A,Bhathena J,Malhotra M. In vitro cytotoxiciy of functionalized single walled carbon nanotubes for targeted gene delivery applications[J].Nanotoxicology,2008.184-188.
  • 9Roelants V,Labar D,de Meester C. Comparison between adenoviral and retroviral vectors for the transduction of the thymidine kinase PET reporter gene in rat mesenchymal stem cells[J].The Journal of Nuclear Medicine,2008,(11):1836-1844.doi:10.2967/jnumed.108.052175.
  • 10Nomura T,Honmou O,Harada K. Ⅰ.Ⅴ.infusion of brain-derived neurotrophic factor gene-modified human mesenchymal stem cells protects against injury in a cerebral ischemia model in adult rat[J].Neuroscience,2005,(01):161-169.

引证文献1

四川大学学报（自然科学版）
2009年 第5期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
关于我们 | 版权声明 | 联系我们 | 帮助中心| 意见反馈
主办单位：上海市教育委员会
技术支持：重庆维普资讯有限公司
渝公网安备 50019002500403号
使用帮助 返回顶部