摘要
目的：优选印楝叶总黄酮的提取工艺条件，并建立总黄酮的含量测定方法。方法：通过正交试验，考察溶剂浓度、回流时间和回流次数对提取工艺的影响。采用分光光度法，以芦丁为对照品，在波长500nm处测定药材总黄酮含量。结果：最佳提取条件为80％的乙醇，回流提取3次，每次1h。总黄酮在0．020～0．080mg／mL范围内线性关系良好（R=0．9992），平均加样回收率为96．67％，RSD为0．96％。结论：优选出的最佳工艺条件经验证其结果稳定；总黄酮含量测定方法简便、快速、稳定，可用于测定印楝叶中总黄酮的含量。
Objective： To optimize extraction technique of total flavonoids and establish a method for measuring the contents of total flavonoids in the leaves of Azadirachta indica. Methods： The optimum process was investigated by L9 （34） orthogonal design. The contents of total flavonoids in the leaves of Azadirachta indica were measured using speetrophotometry with a detection wavelength of 500nm, borrowing the Rutin as a contrast. Results： The optimum technical condition： 80% ethanol, refluxing and extracting for 3 times, one hour each time. The linear range was between 0. 020 ～0. 080mg/mL （R =0. 9992） and the average recovery was 96. 67% with corresponding RSD of 0. 96%. Conclusion： The optimum extraction technology of total flavonoids is stable. The method for determination is simple, rapid and is suitable for determination of total flavonoids in the leaves of Azadirachta indicant.
出处
《成都中医药大学学报》
2009年第3期74-76,80,共4页
Journal of Chengdu University of Traditional Chinese Medicine
基金
四川省教育厅重大培育项目(项目编号:07ZZ006)
关键词
印楝叶
总黄酮
提取工艺
含量测定
The leaves of Azadirachta indica
Total flavonoids
Extracting technology
Content determination
作者简介
范刚，男，1983年生，硕士研究生；
通讯作者：张艺，男，1963年生，教授，研究方向：中药及民族药药效物质基础研究。
