摘要
利用软件Oligo6.0设计1对针对PRRSV NSP2基因的特异性引物,对已检测为PRRSV阳性的病料进行PRRSV NSP2基因扩增及测序。应用DNAstar软件将所测序列与VR-2332毒株、CH-1a毒株(2001)进行比对,发现JXYC与JXNC毒株的核苷酸序列都有87个碱基连续缺失,在与JX2毒株(2007)VR-2332毒株进行比对时发现有相同的缺失,这说明PRRSV已经发生重大变异,而高致病性PRRSV变异不大。该研究补充和丰富了PRRSV毒株的基因组信息数据及PRRSV的分子流行病学的内容。
One pair of specific primers to aim at PRRSV NSP2 gene was designed by software Oligo6.0 and used to PRRSV NSP 2 gene of amplification and sequencing in PRRSV positive cases from swine high fever. The results showed that 87 bases of NSP2 gene of high pathogenic PRRSV had were found continuous deletion by sequence analysis and comparison with virulent strains VR -2332 and CH - 1 a （2001）, the same deletion were found in strain EF014222 -jx by software DNAstar. It showed that PRRSV had much mutated, but High - pathogenic PRRSV had not. The study supply and enrich genome information data and molecular epidemiology contents of PRRSV.
出处
《江西农业大学学报》
CAS
CSCD
北大核心
2009年第4期734-737,共4页
Acta Agriculturae Universitatis Jiangxiensis
基金
江西省科技厅重大招标项目(赣科发计字[2005]93号)
江西省教育厅项目(赣教高字[2008]3号)
作者简介
万根（1973-），男，硕士，主要从事动物传染病及病原学研究
通讯作者：何后军，E—mail：hehoujun@163．com。
