摘要
目的研究穴位和针刺干预对大脑中动脉阻塞(M CAO)缺血模型大鼠脑血流动力作用的特异性。方法将M CAO大鼠分为人中组、内关组、非穴组、未针刺组、模型组,另设正常组和假手术组,每组12只。人中组、内关组和非穴组分别对人中、内关、非穴位部位施以频率3次/s、持续时间5s的针刺干预,观察各组大鼠脑血流量、脑微血管管径和光镜下脑微血管数的变化。结果与未针刺组比较,3个针刺组脑血流量、微血管数量增加明显(P<0.05),微血管管径有相对收缩趋势(P>0.05),表明针刺能明显改善M CAO模型鼠有关脑血流动力的病理状态。3个针刺组间,人中组和内关组比非穴组血流量增多明显、以轻微扩张微血管管径为主(P<0.05),同时人中组还可增加微血管数(P<0.05);在促进脑微血管新生方面人中穴作用强于内关穴。结论针刺穴位产生的效应较非穴位针刺明显,且不同穴位效应是通过特异性作用机制实现的。
Objective To observe the specificity of acupoint and acupuncture in the mechanism of brain-hemodynamies on the model rats with middle cerebral artery obstruction （MCAO）. Methods The MCAO rats were divided into Renzhong （DU26） group, Neiguan （PC6） group, non-acupoint group, non-acupuncture group and model group, 12 rats in each group. And a normal group as well as a sham-operation group were set as control. Renzhong group, Neiguan group, and non-acupoint group were treated with acupuncture in the frequency of 3 times/second for 5 seconds. Then, the effect was observed with the indices of cerebral blood flow （CBF）, cerebral capillary caliber and the quantity of cerebral capillary under the light microscope. Results Compared with non- acupuncture group, in the three acupuncture-treated group, the quantity of CBF and cerebral capillary were increased with significant difference （P〈0. 05）, while the cerebral capillary caliber had the tendency of contraction （P〉0. 05）, indicating that acupuncture could improve pathologic state of brain-hemodynamics of MCAO rats obviously. Of the three acupuncture groups, the CBF increase was more obviously in Renzhong and Neiguan groups than in non-acupoint group, mainly manifested in slightly cerebral capillary caliber extending （P〈0. 05）, meanwhile the quantity of cerebral capillary in Renzhong group increased （P〈0. 05）. Renzhong was better than Neiguan in promoting the production of cerebral capillaries. Conclusion Acu-points acupuncture are more effective than non- acupoint acupuncture in the improvement of brain-hemodynamics on rats with MCAO, and different acupoints have different effects through their specificity.
出处
《中医杂志》
CSCD
北大核心
2009年第8期712-715,共4页
Journal of Traditional Chinese Medicine
基金
国家重点基础研究发展计划(“973”计划)项目(2006CB504504)
关键词
针刺
穴位特异性
血流动力学
脑梗死
大脑中动脉阻塞(MCAO)
大鼠
Acupuncture
Acupoint specificity
Hemodynamics
Cerebral infarction
Middle cerebral artery obstruction （MCAO） Rat
作者简介
通讯作者：石学敏，tchosa@mailzlnet．com．cn，（022）27432008
