摘要
目的：驱虫斑鸠菊是治疗白癜风的常用药材,其化学成分复杂。文中建立并优化驱虫斑鸠菊高效液相色谱指纹图谱（HPLC-FP）的色谱条件,并在此基础上进行不同提取工艺的对比研究。方法：通过保留时间及各共有峰的相对峰面积建立并优化分析驱虫斑鸠菊组分的高效液相色谱指纹图谱（HPLC-FP）,进行不同提取工艺的对比研究。结果：建立了驱虫斑鸠菊最优的HPLC-FP分离条件,得到19个指纹图谱共有峰,经对比得知驱虫斑鸠菊85%乙醇提取物与半仿生提取物的指纹图谱比较相似,而与驱虫斑鸠菊水提物指纹图谱差异较大,其中1、4、6、11、12、14、16-19峰为驱虫斑鸠菊85%乙醇提取物及驱虫斑鸠菊半仿生提取物的高效液相色谱指纹图谱特有峰,而20-30峰则为驱虫斑鸠菊水提取物的高效液相色谱指纹图谱特有峰。结论：实验为驱虫斑鸠菊入血成分、制剂工艺及药效学研究提供实验依据。
Objective： Vernonia anthelmintica, with complicated components, is a common medicine for the treatment of vitiligo. The purpose of this study was to establish and optimize the separation conditions of high performance liquid chromatography fingerprints （HPLC-FPs） for Vernonia anthelmintica （L.） Willd. Methods： We investigated the HPLC-FPs of different extraction samples by the retention times and relative areas of common peaks in the fingerprints. Results ： We established the optimum separation conditions of HPLC-FPs for Vernonia anthelmintica （L.） WiUd, and obtained 19 common peaks in the fingerprints. The HPLC-FPs of the 85% ethanol extract were quite similar to those of the semi-bionic extract （SBE） but very different from those of the water extract of Vernonia anthelmintica （L.） Willd. The No. 1,4, 6, 11, 12, 14 and 16 -19 peaks were the endemic ones of the HPLC-FPs of the 85% ethanol extract and SBE of Vernonia anthelmintica （L.） Willd, while No. 20 -30 were the endemic peaks of the HPLC-FPs of the water extract. Conclusion ： The results of our study has provided experimental evidence for further investigation into the constituents absorbed into blood, pharmaceutical technology and pharmacodynamics of Vernonia anthelmintica （ L. ） Willd.
出处
《医学研究生学报》
CAS
2009年第8期794-798,共5页
Journal of Medical Postgraduates
基金
国家科技支撑计划基金资助项目(批准号:2007BAI30B01
2007BAI48B06)
关键词
驱虫斑鸠菊
高效液相色谱
指纹图谱
工艺对比
Vernonia anthelmintica （L.） Willd
High performance liquid chromatography
Fin-gerprint
Process comparison
作者简介
刘晓东（1980-），男，河南商丘人，医学硕士研究生，从事药学研究专业。
闫明（1959-），男，山两原平人，研究员，医学硕士，从事药学专业。E—mail：yanming21cn@sohu．com
