摘要
在厌氧、37℃和120 r/min条件下,将味精废水接种活性污泥后进行批培养,向发酵液中添加乳酸盐(终浓度为0.05 g/L),不仅补偿了F420分子合成前体的非均衡性供给,而且提高了厌氧电子传递效率,促进了甲烷菌群细胞的镍同化作用(同化镍含量比对照组提高了68.8%),从而最终提高了味精废水发酵产气效率。试验组(添加乳酸盐)挥发性乙酸盐残留量比对照组减少了62%,沼气总产量、总固形物TS、消耗单位质量挥发性脂肪酸的沼气得率系数YP/V、单位质量总固形物的沼气得率系数YP/T、挥发性脂肪酸的比消耗速率qV、沼气的比生成速率qp分别比对照组提高了48.8%,12.5%,21.9%,31.4%,13.8%和31.2%,说明向味精废水中添加乳酸盐,可以促进挥发性乙酸钠底物代谢流向终产物甲烷而非仅用于菌群繁殖,乳酸盐是通过提高菌群的甲烷合成能力,而非仅通过促进菌群生长的群体优势来提高沼气产量。
The effects of lactate addition in glutamate wastewater for methane production during the anaerobic batch fermentation were investigated at the condition of 37 ℃ and 120 r/min. Lactate addition of 0.05 g/L compensated the unbalanceable supply of precursors used for the biosynthesis of coenzyme F420 and increased the efficiency of electron transfer under anaerobic fermentation condition, thus enhancing the assimilation of Ni （concentration of Ni was 68.8% higher than the control group）. The residual volatile acetate is 62% lower than the control group, The total yield of biogas, TS, YP/V （coefficient of biogas yield to the consumed volatile fatty acid）, YP/T （coefficient of biogas yield to the total solids）, qV （unit consumption rate of volatile fatty acid）, qp （unit production rate of biogas） has increased by 48.8%, 12.5%, 21.9%, 31.4%, 13.8% and 31.2% respectively by comparison with the control group. This means that the lactate addition in glutamate wastewater may facilitate the metaboly of the substrate of volatile sodium acetate except for increasing the microorganism amounts, the lactate addition will increases the biogas yield through improving the synthesis capability for methane instead of through increasing the microorganism amounts.
出处
《可再生能源》
CAS
北大核心
2009年第4期24-27,共4页
Renewable Energy Resources
基金
武汉市科学技术局攻关项目(200820212029)
湖北省教育厅重点项目(D20081401)
湖北工业大学博士启动基金(20070021)
关键词
味精废水
厌氧发酵
沼气
glutamate wastewater
anaerobic batch cultivation
biogas
作者简介
王志（1971-），男，博士，主要从事发酵工程与生物质能利用技术的研究工作。E—mail：wangzhi1002@126．com
通讯作者：王金华（1964-），男，教授，主要从事生物技术及生物质能利用技术的研究工作。E-mml：jinhwang99@126．com
