摘要
目的:了解异丙酚对大鼠脑一氧化氮合酶(NOS)活性和一氧化氮(NO)产量的影响。方法:16只SD大鼠,随机分为二组,分别IP生理盐水10ml·kg^(-1)(对照组)和异丙酚100mg·kg^(-1)。待翻正反射消失后立即断头,0℃～4℃取脑,分区,匀浆,离心制备10%组织匀浆液,用分光光度法测定NOS活性和NO产量。结果:与对照组比较,大鼠IP异丙酚100mg·kg^(-1)不但明显抑制小脑、大脑皮层、海马和脑干的NOS活性,而且显著减少上述脑区的NO产量(P<0.05或P<0.01)。结论:NO可能在异丙酚全麻分子学机理中发挥重要作用。
Objective:To investigate effects of propofol on nitric oxide synthase (NOS) activity and nitric oxide (NO)output of rat brain. Method: Sixteen SD rats were divided randomly into two groups. The animals were administered introperitoneally(ip) normal saline 10 ml·kg^(-1)(control group)or propofol 100mg·kg^(-1)(propofolgroup),respectively. These rats were decapitated immediately after having disappeared righting reflex. After rapid removal of cerebellum, brain stem,hippocampus and cerebral cortex,tissues were homogenized and centrifuged. NOS activity and NO output were assayed with spectrophotometric analysis. Result: In propofol group,NOS activity was significantly inhibited, NO outpul was significantly reduced in cerebellum, brain stem,hippoeampus and cerebral cortex as compared with those of control group(P<0.05 or P<0.01)Conclusion:NO may play a important role in tbe molecular mechanism of propnfoI anesthesia.
出处
《中华麻醉学杂志》
CAS
CSCD
北大核心
1998年第7期427-428,共2页
Chinese Journal of Anesthesiology
基金
江苏省教委自然科学基金课题:No96093
