摘要
目的观察急性百草枯（PQ）中毒大鼠肾损伤病理变化和血红素氧合酶-1（HO-1）的表达，探讨PQ中毒。肾损伤病理生理机制。方法sD大鼠随机分为对照组和染毒组，染毒组予25mg／kgPQ一次性腹腔注射染毒，对照组等量盐水腹腔注射。分别观察3、6、12h和1、2、3、5d时肾组织病理学变化，采用免疫组织化学法观察血红素酶（HO．1）蛋白表达，反转录一聚合酶链反应（RT-PcR）检测HO-1mRNA表达。结果（1）对照组肾组织结构清晰，染毒组肾组织结构清晰度下降，染毒3h即可见充血、水肿及空泡变性等病理变化，随时间延长而加重；重者可见核固缩，细胞结构消失，但其范围并不随时间延长而增加。肾小球、髓质部亦可受累。对照组HO-1蛋白与HO-1mRNA多不表达；染毒组染毒3h在皮质部肾小管上皮细胞的胞膜及胞浆中HO-1蛋白呈阳性表达，免疫组织化学评分（IHS）升高，与对照组相比，除染毒5d外其他各时间点比较，差异均有统计学意义（P〈0．05）；除染毒3、5d外，其他时间点HO-1mRNA表达分别为（0．53±0．21）、（0．55±0．31）、（0．56±0．22）、（0．64±0．14）、（0．43±0．25），明显高于对照组，差异均有统计学意义（P〈0．05）。结论PQ中毒肾损伤病理改变可能存在不同作用机制；HO-1蛋白及HO-1mRNA高表达提示其可能参与PQ中毒肾损伤病理生理过程。
Objective To study the mechanism of paraquat-induced renal injury in rats. Methods Adult healthy Spragne-Dawley （SD） rats （female and male in half） were randomly divided into two groups, the con- trol group and the paraquat poisoned group. The rats in the paraquat poisoned group were treated with PQ （25 mg/kg） intraperitoneally while the rats in the control group were treated with the same dose of normal salineas. Its histopathological change was observed and the expression of HO-1 and the mRNA expression of HO-1 were detected by RT-PCR at 3rd h, 6th h, 12th h, on 1st d, 2nd d, 3rd d and 5th d. Results （ 1 ）In the control group, the tissue structure was clear without edema, vacuolar degeneration, cloudy swelling and necrosis. In the paraquat poisoned group, there were obvious lesions in the renal tubule of cortical part, including cellular swelling, the narrow canula, the mesenchymal congestion and edema. These pathologic changes gradu- ally became more severe, reached the peak on the 1st day, and did not relieve until the end of this study; there was the karyopycnosis and the cyto-architecture disappeared in some severe cases; Some glomerulus and medulla were also involved. （2）In the control group, there was no or weak expression of HO-1 and HO-lmR- NA. At the 3rd hour, the expressions of HO-1 in the paraquat poisoned group were observed in the membrane and kytoplasm of renal tubular epithelial cell of cortical part. Immunohistochemistry score （IHS） in the paraquat poisoned group was higher than that in the control group （P〈0.05）, except the HIS of the 5th day. At the 3rd hour, the expression of HO-lmRNA increased, reached the peak on the 1st day, and then decreased. The expression of HO-lmRNA was （0.53±0.21）, （0.55±0.31）, （0.56±0.22）, （0.64±0.14） and （0.43±0.25） at the time point other than on the 3rd and 5th day. It showed statistical difference between the paraquat poisoned group and the control group from the 3rd hour to the 2nd day （P〈0.05）. Conclusion The mechanism of paraquat induced-renal injury is multiple. The higher expression of HO-1 and HO-lmRNA were involved in the procedures of paraquat-induced renal injury.
出处
《中华劳动卫生职业病杂志》
CAS
CSCD
北大核心
2009年第8期468-471,共4页
Chinese Journal of Industrial Hygiene and Occupational Diseases
