摘要
根据GenBank公布的的SIP基因序列,设计一对特异性引物,通过对PCR方法的优化,建立了牛无乳链球菌性乳房炎的快速PCR检测方法。用建立的PCR检测方法对患牛乳中无乳链球菌总DNA进行扩增,获得大小为945bp的DNA片段。特异性试验表明,停乳链球菌、金黄色葡萄球菌、大肠杆菌DNA均未扩出条带;敏感性试验表明,该对引物能够检测到的最低DNA浓度为1.25ng;重复试验表明该方法具有良好的重复性和稳定性。
According to surface immunogenic protein gene necleotide sequence from Genbank, a pair of specific primers were designed and PCR conditions were optimized, then a PCR rapid determination method for streptococcus agalactiae in milk was established. This study aehived DNA with 945bp in length, as the sample of total DNA in milk from mastitis dairy cow. The result of specificity assay showed that the control samples for Streptococcus dysgalactiae DNA, Staphytococcus aureus DNA were negative, as well as Escherichia coli DNA. The result of sensitivity assay showed that the detected density of DNA at least was 1.25ng. The result of repeat test showed that this established method has good repeatability and stability.
出处
《中国奶牛》
2009年第8期17-19,共3页
China Dairy Cattle
基金
黑龙江省农业科学院博士后基金项目资助(LRB07-118)
关键词
奶牛乳房炎
无乳链球菌
PCR
诊断
Dairy rnastitis
Streptococcus agalactiae
PCR
Diagnosis
作者简介
布日额（1962-），男，蒙古族，内蒙古通辽市人，教授，博士，硕士研究生导师．主要从事微生物分子生物学与生物技术研究。
