摘要
利用冰冻切片法结合间接免疫荧光标记技术对割手密茎尖细胞有丝分裂过程中微管骨架的变化进行了研究。结果表明:在割手密茎尖细胞有丝分裂过程中存在4种循序变化的典型微管列阵,即周质微管、早前期微管带、纺锤体微管及成膜体微管。在割手密初生增粗分生组织细胞中观察到的大多数是周质微管列阵,很少观察到其它3种典型的微管列阵,这可能这是割手密茎较小的原因之一。
The mierotubule cytoskeleton was investigated by using cryosectioning and indirect immunofluorescence staining in the shoot apex of Saccharum spontaneurn. The results indicated that: there were four basic types of microtubule arrays:cortical microtubule,preprophase-band,spindle and phragmoplast microtubule arrays. Cortical microtubule arrays were predominant in the primary thickening meristem of S. spontaneum, while other three arrays were rarely observed. It may be one of the reasons why S. spontaneum has a narrower stem than other cultivars.
出处
《广西植物》
CAS
CSCD
北大核心
2009年第4期497-501,共5页
Guihaia
基金
国家自然科学基金(30060038)
国家科技支撑计划项目(2008BADB8B00)
广西科学基金(0991048)~~
关键词
割手密
初生增粗分生组织
微管骨架
有丝分裂
Saccharum spontaneum
primary thickening meristem
microtubule eytoskeleton
mitosis
作者简介
通讯作者:李志刚(1969-),男.广西蒙山人,副教授,从事甘蔗解剖生理及环境生态研究,(E-mail)lizhigangnn@163.com。