摘要
以槐耳菌丝体生物量为主要指标,采用不同碳源和氮源进行单因素初选试验,然后以不同比例的混合碳氮源进行正交试验.结果表明,槐耳菌丝体液体最佳培养基是玉米渣200 g/L,蔗糖20 g/L,酵母膏3 g/L,麦麸3.5 g/L,KH2PO42.0 g/L,MgSO4.7H2O 1.0 g/L和VB16 mg/L,温度28℃,pH自然.其菌丝体生物量得率为21.6 g/L.
Biomass of mycelium in liquid culture medium for Trametes robiniophila Murr. as major index, firstly different carbon source and nitrogen source were adopted to early out the single factor primary election in the experiment, carrying out tests again for different proportional commixture of carbon source and nitrogen source. The results showed that the optimal medium was leaching solution of corn 200 g/L, cane sugar 20 g/L, yeast extract 3 g/L, wheat bran 3. 5 g/L, KH2PO4 2.0 g/L, MgSO4 · 7H2 O 1.0 g/L, VB1 6 mg/L with 28 ℃, natural pH. The production rate of biomass on mycelium of Trametes robiniophila Mutt. reached 21.6 g/L in the liquid culture medium.
出处
《天津师范大学学报（自然科学版）》
CAS
北大核心
2009年第3期66-68,共3页
Journal of Tianjin Normal University：Natural Science Edition
基金
天津市科委自然科学基金项目(06ZHCXNC05900)
关键词
槐耳
液体培养基
菌丝体
生物量
正交设计
Trametes robiniophila Murr.
liquid culture medium
mycelium
biomass
orthogonal design
作者简介
杨子美（1984-），女，硕士研究生．
通讯作者：郭成金（1952-），男，副教授，主要从事植物营养生理与蕈菌生物学的研究．
