摘要
为了简便高效的制备出壳寡糖,采用过氧化氢(质量分数1.0%)来降解稀醋酸溶液(质量分数1.0%)中含量为3.5%的壳聚糖,60℃下摇床反应4.5h后得到壳寡糖水溶液,检测溶液中还原性端基数并估算降解物平均聚合度来表征降解程度。降解液浓缩到25%,调pH7.0,用体积分数95%乙醇分级沉淀分离壳寡糖可以除掉部分单糖,总的回收率达93.0%。通过电喷雾电离质谱(ESI-MS)分析,3倍醇沉出的产物为2-4糖为主的壳寡糖产品。实验说明过氧化氢氧化降解壳聚糖可制取聚合度为2-4的壳寡糖。
Low molecule weight chitosan oligosaccharide （COS）was prepared by degraded 3.5% chitosan in 1.0% acetic acid with 1.0% hydrogen peroxide at 60℃ shaking for 4.5 hours. Concentration was then adjusted to 25% , pH 7.0. The solution finally was precipitated by 95% alcohol and COS was obtained without monosaccharide at the yield of 93.0%. The molecular weight was estimated by Fehling oxidation-reduction titration which was detecting the average value of the reducing ends. The distribution of COS was analyzed by electro-spray ionization mass spectromotry （ESI-MS）. The COS comprised mainly of 2 to 4 chitosan oligosaccharides was precipitated by three volume of alcohol. The conclusion was that COS could be efficiently prepared by the hydrogen peroxide oxidation of chitosan.
出处
《食品与发酵工业》
CAS
CSCD
北大核心
2009年第6期121-125,共5页
Food and Fermentation Industries
关键词
壳寡糖
过氧化氢
降解
醇沉
chitosan oligosaccharide, hydrogen peroxide, degradation, alcohol precipitation
作者简介
第一作者：硕士研究生
钱俊青教授为通讯作者
