摘要
应用紫外-可见光谱法和荧光光谱法并以中性红(NR)作光谱探针研究了Sm(Ⅲ)HP配合物与DNA的作用机制。研究表明,血卟啉(HP)与Sm(Ⅲ)的配合比nSm(Ⅲ)∶nHP=1∶1,Sm(Ⅲ)HP配合物与DNA的结合比nSm(Ⅲ)HP∶nDNA=1∶1,Sm(Ⅲ)HP配合物与DNA的结合常数K3θ0℃=1.67×104L/mol。该过程为熵所驱动,其ΔrHm为4.86×104J/mol,ΔrSm为195.61 J/(mol.K),30℃时ΔrGm为-1.06×104J/mol。Scatchard法和探针法研究表明,Sm(Ⅲ)HP与DNA之间的结合方式主要是沟区作用。
Using neutral red （NR） as a probe, functionary mechanism between Sm （ Ⅲ ） HP and DNA was studied by UV-vis and fluorescence spectroscopy. The results indicated that the binding ratio of nsm（Ⅲ） ： nHP is 1 ： 1, nsm（Ⅲ）HP： nDNA =1 ： 1, and the binding constant of Sm （ Ⅲ ） HP complex with Herring Sperm DNA was K30℃^θ = 1.67 × 10^4 L/mol. Thermodynamic parameters of enthalpy （△rHm^θ）, entropy （△rSm^θ） , and Gibbs free energy （△rGm^θ） were calculated to be 4. 86 × 104 J/mol, 195.61 J/ （ mol · K） and - 1.06 × 104 J/mol, respectively. The results suggested that the process of interaction of Sm （ Ⅲ ） HP complex and Herring Sperm DNA was driven by entropy. The results of scatchard and probe methods suggested that the primary interaction mode between Sm （ Ⅲ ） HP and DNA was groove binding.
出处
《中山大学学报（自然科学版）》
CAS
CSCD
北大核心
2009年第4期61-65,共5页
Acta Scientiarum Naturalium Universitatis Sunyatseni
基金
国家自然科学基金资助项目(30572254)
作者简介
赵娜（1983年生）女，硕士研究生；通讯作者：王兴明；E-mail：xmwang_xkd@yahao．cn
