摘要
目的分析沙门菌质粒DNA上整合子的分子特征。方法用全自动细菌分析仪Microscan WalkAway40和纸片扩散法,对32株沙门菌进行抗生素敏感性测定,提取细菌质粒DNA,PCR扩增I类整合子、II类和III类整合酶,对扩增产物进行序列分析。结果12株细菌含有一个I类整合子,整合子大小分别为1000bp和1700bp。1000bp整合子含基因盒aadA1,1700bp整合子含基因盒dfr17-aadA5,未检测到II类和III类整合酶。结论整合子在沙门菌中广泛存在,整合子是介导和传播细菌耐药性的重要分子机制。
Objective To analyze the molecular feature of integrons on plasmid DNA in Salmonella. Methods The susceptibility of 32 Salmonella isolates to antibiotics were tested by Microsean WalkAway40 and the disk diffusion assay. Plasmid DNA was extracted. Class 1 integrons,intI2 and intI3 were detected by PCR. The purified amplicons of class 1 integrons were sequenced,and gene cassettes were identified. Results There 12 isolates carried class 1 integrons on plasmid DNA. The sizes of class 1 integrons were 1 000bp and 1 700bp respectively. 1 000bp integron carried gene cassette aadA1,1 700bp integron carried gene cassette dfr17-aadAS. There were no IntI2 and intI3. Conclusion Integrons are widely carried by Salmonella. Integrons are important molecular mechanism in mediation and dissemination of antibiotic resistance in Salmonella.
出处
《中国热带医学》
CAS
2009年第8期1401-1401,1595,共2页
China Tropical Medicine
关键词
沙门菌
整合子
基因盒
耐药
Salmonella
Integrons
C, ene cassettes
Resistance to antibiotics
作者简介
蔡俊源（1963～），大专，主管检验技师，主要从事卫生检验工作
