摘要
目的观察三苯双脒、青蒿琥酯和吡喹酮对感染华支睾吸虫金色仓鼠的疗效。方法93只仓鼠各感染30个华支睾吸虫囊蚴,分组后灌胃顿服给药治疗,观察各组疗效。①31只感染仓鼠中,20只于感染后14d随机均分为4组,分别为青蒿琥酯300mg/kg组、三苯双脒100mg/kg和200mg/kg组、吡喹酮200mg/kg组,观察药物对华支睾吸虫童虫的作用;另6只于感染后24d随机均分为2组,分别为三苯双脒200mg/kg组和青蒿琥酯300mg/kg组;余5只作对照组。②22只仓鼠于感染后28d随机分成5组(每组4～5只),分别为三苯双脒25mg/kg和50mg/kg组、青蒿琥酯25mg/kg组、吡喹酮50mg/kg组,以及对照组。③40只仓鼠于感染后28d随机分成8组(每组4～6只),分别为三苯双脒50mg/kg、100mg/kg和200mg/kg组,青蒿琥酯100mg/kg和200mg/kg组,吡喹酮100mg/kg和200mg/kg组,以及对照组。各组受治鼠于治疗后2周剖杀,收集胆道系统内的残留华支睾吸虫,计算各组的平均虫数和减虫率。结果仓鼠感染华支睾吸虫囊蚴后14d,三苯双脒100mg/kg和200mg/kg组,以及吡喹酮200mg/kg组的平均虫数均显著低于对照组(P<0.05),减虫率分别为90.6%、85.9%和71.9%;青蒿琥酯300mg/kg组平均虫数与对照组差异无统计学意义(P>0.05)。感染后24d,童虫已发育为成虫,三苯双脒200mg/kg组平均虫数显著低于对照组(P<0.01),减虫率为89.8%;青蒿琥酯300mg/kg组的减虫率为100%。感染后28d,三苯双脒25mg/kg组平均虫数显著低于对照组(P<0.05),减虫率为71.8%,剂量增至100mg/kg时,减虫率为100%;青蒿琥酯25mg/kg和100mg/kg组的减虫率分别为20.0%和56.4%,剂量增至200mg/kg时,减虫率为98.5%,其平均虫数显著低于对照组(P<0.01)。吡喹酮100mg/kg和200mg/kg组的减虫率分别为78.9%和83.5%,其平均虫数均与对照组的差异有统计学意义(P<0.05)。结论三苯双脒和吡喹酮对感染华支睾吸虫童虫和成虫的仓鼠均有较好的疗效,青蒿琥酯仅对成虫有效。
Objective To observe the effect of tribendimidine, artesunate and praziquanel in treatment of hamsters （Mesocricetus auratus） infected with Clonorchis sinensis. Methods A total of 93 hamsters, each infected with 30 C. sinensis metacercariae, were treated intragastrically with above-mentioned drugs at a single dose. （1） In order to observe the effect of the drugs against juvenile C. sinensis, 20 out of 31 infected hamsters were randomly divided into 4 groups （5 hamsters per group） 14 d post-infection： artesuante 300 mg/kg, tribendimidine 100 mg/kg or 200 mg/kg, and praziquantel 200 mg/kg. Other 6 hamsters were divided equally into 2 groups 24 d post-infection and treated with tribendimidine 200 mg/kg and artesunate 300 mg/kg, respectively. The remained 5 untreated hamsters served as control. （2） Twenty-two hamsters were randomly divided into 5 groups （4-5 hamsters per group） 28 d post-infection and treated with tribendimidine 25 mg/kg or 50 mg/kg, artcsunate 25 mg/kg and praziquantel 50 mg/kg, respectively. Other untreated hamsters served as control. （3） Forty hamsters 28 d after infection were randomly divided into 8 groups （4-6 hamsters per group） and treated with tribendimidine 50 mg/kg, 100 mg/kg or 200 mg/kg, artesunate 100 mg/kg or 200 mg/kg,praziquantel 100 mg/kg or 200 mg/kg, respectively. The remained hamsters served as control. All hamsters were sacrificed 14 d post-treatment and worms were recovered from the bile duct and liver tissue. The mean worm burden and its reduction were calculated. The differences of mean worm burden between each treated group and the corresponding control were analyzed statistically. Results In hamsters infected with 14-d-old C. sinensis and treated orally with tribendimidine at a single dose of 100 or 200 mg/kg, the mean worm burdens were significantly lower than that of the control （P〈0.01） with a worm reduction of 90.6% and 85.9% respectively. The mean worm burden obtained from the infected hamsters treated with praziquanel at a single dose of 200 mg/kg was also significantly lower than that of the control （P〈0.05） with a worm reduction of 71.9%. However, the difference of mean worm burden between artesunate and control groups was not statistically significant. The juvenile parasites developed into adult worms 24 d after infection. By administering tribendimidine 200 mg/kg to the adult C. sinensis-infected hamsters, the mean worm burden was significantly lower than that of the control （P〈0.01） with a worm reduction of 89.8%. Whilst the administration of artesunate at a higher dose of 300 mg/kg, all hamsters were cured. Further tests indicated that tribendimidine in a lower dose of 25 mg/kg to the hamsters 28 d after infection resulted in a significantly lower mean worm burden compared to the control （P〈0.05） with a worm reduction of 71.8%. With an increased dose of tribendimidine 100 mg/kg, all hamsters were cured, The worm reduction was only 20.0% and 56.4% when 25 mg/kg and 100 mg/kg of artesunate were administered. With 200 mg/kg artesunate, the worm reduction reached as high as 98.5% and the mean worm burden was significantly lower than that of the control （P〈0.01） . Furthermore, administration of praziquantel at a dose of 100 mg/kg or 200 mg/kg at 28 d post-infection resulted in a significantly lower mean worm burden than that of the control （P〈0.05） with a worm reduction of 78.9% and 83.5% respectively. Conclusion In hamster model, tribendimidine and praziquantel exhibit promising effect against both juvenile and adult C. sinensis, while artesunate is only efficacious against adult worms.
出处
《中国寄生虫学与寄生虫病杂志》
CAS
CSCD
北大核心
2009年第3期215-218,共4页
Chinese Journal of Parasitology and Parasitic Diseases
基金
中国疾病预防控制中心寄生虫病预防控制所资助
关键词
华支睾吸虫
金色仓鼠
三苯双脒
吡喹酮
青蒿琥酯
疗效
Clonorchis sinensis
Mesocricetus auratus
Tribendimidine
Praziquantel
Artesunate
Efficacy
作者简介
通讯作者．E—mail：shxiao4@hotmail．com
