摘要
目的:探讨不同浓度超顺磁性氧化铁(SPIO)颗粒标记鼠骨髓间充质干细胞(MSCs)的标记率和对细胞活力的影响,以及MR成像显示磁标记干细胞的可行性。材料和方法:将不同浓度的SPIO-PLL复合物与培养基混合,行普鲁士蓝染色观察细胞内铁和检测细胞活力。应用1.5TMR仪,以T1WI和T2WI行磁标记干细胞成像。结果:SPIO可有效标记MSCs,标记后的铁颗粒位于细胞质内。SPIO标记的MSCs可引起T2WI信号降低,50、100、150较25μgFe/ml的信号强度降低明显。结论:SPIO可以简便标记MSCs,并在适当浓度下对细胞活力没有影响,此技术为干细胞移植的MR活体内示踪奠定基础。
Purpose： To explore the labeling efficiency and cellular viability of rat bone marrow mesenchymal stem cells labeled with different concentration of superparamagnetic iron oxide （ SPIO ） particles, and to determine the feasibility of detection of magnetically labeled stem cells with MR imaging. Materials and Methods： The culture media containing SPIO - PLL of different concentration were added to the MSCs. Prussian blue staining for demonstrating intracytoplasmie nanopartieles and cell viability were performed respectively after labeling. MR imaging of cell suspensions was performed by using T1WI and T2WI sequences at a clinical 1.5 T MR system. Results： MSCs were efficiently labeled with SPIO, labeled SPIO particles were stained in cytoplasm. SPIO caused a stronger low signal attenuation effect on T2WI, the signal intensity of 50,100,150μgFe/ml is lower than 25 μgFe/ml. Conclusion： MSCs can be easily and efficiently labeled by SPIO without interference on the cell viability, MRI visualization of SPIO labeled MSCs is feasible, which may be critical for future experimental studies.
出处
《中国医学影像学杂志》
CSCD
北大核心
2009年第4期257-261,共5页
Chinese Journal of Medical Imaging
关键词
干细胞
超顺磁性氧化铁
MRI
stem cells
superparamagnctic iron oxid
magnetic resonance imaging
